Vitamin K alleviates bone calcium loss caused by Salmonella Enteritidis through carboxylation of osteocalcin  

作　　者：Yaojun Liu Rainer Mosenthin Lihong Zhao Jianyun Zhang Cheng Ji Qiugang Ma 

机构地区：[1]State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China [2]Institute of Animal Science,University of Hohenheim,70593 Stuttgart,Germany

出　　处：《Journal of Animal Science and Biotechnology》2021年第4期1572-1581,共10页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Science Foundation of China(Grant No.31772627);a Special Fund for China Agricultural Research System program(CARS-40-K08);the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation,328017493/GRK 2366);National Key Research and Development Program of China(2017YFD0500500);National Science Foundation of China(Grant No.31772627);was funding for the design of the study and collect sample.A Special Fund for China Agricultural Research System program(CARS-40-K08)was funding for writing the manuscript;Deutsche Forschungsgemeinschaft(DFG,German Research Foundation,328017493/GRK 2366);National Key Research and Development Program of China(2017YFD0500500)were funding for analysis the sample.

摘　　要：Background:The present study aimed at evaluating the effect of vitamin K(VK)supplementation on bone health of laying hens challenged by Salmonella Enteritidis.Methods:A total of 8032-week-old double negative salmonella-free brown-egg laying hens were randomly assigned to 4 treatments with 20 replicates each(1 bird per replicate)according to a 2×2 factorial design with 2 dietary VK supplementation levels[0 mg/kg(VK0)vs 2 mg/kg VK(VK2)and 2 challenge treatments[Salmonella Enteritidis(SE)vs physiological saline solution(PS)].During the last 3 days of week 43 of age,birds of both VK treatments were either orally challenged with 1.0 mL suspension of 109 cfu/mL S.Enteritidis daily or received the same volume of PS.Results:The laying rate,daily egg mass,tibia strength,CT,cOC and cOC/(cOC+ucOC)of VK2 treatment increased(P<0.05)in contrast to VK0,however,the medullary area and ucOC of VK2 treatment decreased(P<0.05)in contrast to VK0.Mortality,medullary area,serum Ca content of SE treatments increased(P<0.05)in contrast to PS treatments.In both SE treatments,the decrease(P<0.05)in birds’tibia strength was associated with higher(P<0.05)Ca levels in serum.There is an interaction(P<0.05)between SE challenge and VK levels with regard to tibia strength and serum Ca levels.At week 42,serum CT was positively correlated with cOC(R=0.99,P=0.009);at week 44,tibia strength was positively correlated with BMD(R=0.95,P=0.045),but negatively correlated with medullary area(R=−0.98,P=0.018).Conclusions:VK(2 mg/kg)supplementation to diets of laying hens can enhance bone strength under challenge situations with Salmonella Enteritidis.Medullary area has proven to be a sensitive biomarker for bone calcium loss caused by SE infection.

关 键 词：CALCIUM Laying hen Salmonella Enteritidis Vitamin K 

分 类 号：S858.31[农业科学—临床兽医学]