UV-B辐射对84K杨酚酸类化合物积累及其生理生化的影响  

Effects of UV-B Radiation on Phenolic Acid Accumulation and Physiological and Biochemical Characters in 84K Poplar

作　　者：盖庆岩[1] 王紫莹 付玉杰[1] 焦骄[1] 王慧梅[1] 鹿瑶 刘婧[1] 富锦先 徐晓杰 姚兰 GAI Qing-Yan;WANG Zi-Ying;FU Yu-Jie;JIAO Jiao;WANG Hui-Mei;LU Yao;LIU Jing;FU Jin-Xian;XU Xiao-Jie;YAO Lan(College of Chemistry,Chemical Engineering and Resource Utilization,Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学化学化工与资源利用学院,森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2021年第6期878-887,共10页Bulletin of Botanical Research

基　　金：“十三五”国家重点研发计划项目(2017YFD0600205)。

摘　　要：UV-B辐射作为一种典型的环境胁迫因子,可对植物体的生长发育和生理代谢产生显著的影响,因此研究材用树种杨树对于UV-B辐射的防御性响应具有重要的意义。以84K杨组培苗为实验对象,对其进行不同程度的UV-B辐射胁迫,分析胁迫处理前后84K杨组培苗中6种防御性酚酸类化合物的含量变化规律,并从光合色素和ROS系统角度探讨目标酚酸类化合物的积累机制。研究结果表明:适当的UV-B胁迫处理有助于5种目标酚酸类化合物(阿魏酸、咖啡酸、原儿茶酸、绿原酸和异阿魏酸)的合成和积累,尤其是绿原酸在辐射8 h时含量增加了15倍左右;光合色素含量变化与酚酸类化合物趋势一致,在16 h时总叶绿素含量提高了1.21倍;抗氧化酶(尤其是POD)与酚酸类化合物协同抵御了由UV-B辐射而引起的氧化应激。本研究对于阐明杨树在UV-B辐射胁迫下防御性酚酸类化合物的代谢调控机制提供了一定的理论依据。As a typical environmental stress factor,UV-B radiation could make a significant impact on the growth,development,and physiological metabolism of plants,and it could be of great significance to understand the defensive response of wood species poplar to UV-B radiation.In this study,84K poplar seedlings used as materials were subjected to different intensities of UV-B radiation.The contents of six defensive phenolic acids in 84K poplar seedlings before and after the stress were analyzed,and the accumulation mechanism of target compounds was discussed from the perspective of photosynthetic pigments and ROS system.The results indicated that the proper UV-B treatment was helpful for the accumulation of five target phenolic acids(ferulic acid,caffeic acid,protocatechuic acid,chlorogenic acid and isoferulic acid),especially the content of chlorogenic acid increased about 15 times after 8 h of UV-B radiation;the accumulation trend of photosynthetic pigments was the same as phenolic acids,and the content of total chlorophyll increased 1.21 times after 16 h of UV-B radiation;antioxidant enzymes(especially POD)were found cooperate with phenolic acids to resist the oxidative stress caused by UV-B radiation.Overall,this study provided a theoretical basis for elucidating the metabolic regulation mechanism of defensive phenolic acids in poplars under UV-B radiation stress.

关 键 词：杨树 UV-B辐射 酚酸类化合物 光合色素 ROS 

分 类 号：S792.11[农业科学—林木遗传育种]