反硝化细菌的筛选及菌藻共培养体系除氮特性  

Screening of Aerobic Denitrifying Bacteria and Nitrogen Removal Characteristics of Bacteria-algae Co-culture System

作　　者：肖思远 朱文娟 陈思宇 兰时乐[1] XIAO Siyuan;ZHU Wenjuan;CHEN Siyu;LAN Shile(College of Biological Science and Technology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《环境科学与技术》2021年第8期154-162,共9页Environmental Science & Technology

基　　金：湖南省重点研发项目:池塘养殖水质净化新型微生态制剂研究与开发(2016NK2103)。

摘　　要：为提高养殖废水的除氮效果,该文从精养池塘水体中分离出1株高效好氧反硝化细菌TC-1,通过形态学观察、生理生化试验并结合16S r DNA序列分析对反硝化细菌TC-1进行了鉴定。选取椭圆小球藻与反硝化细菌TC-1为研究对象,运用菌藻共培养法研究了碳源种类、总接种量、初始p H值、培养温度、摇床转速对菌藻共培养体系除氮性能的影响,并应用Box-Behnken中心组合进行3因素3水平的实验设计对培养条件进行优化。结果表明,菌株TC-1被鉴定为Pseudomonas toyotomiensis TC-1。菌藻共培养体系适宜的脱氮条件为:在以丁二酸钠为碳源,总接种量2%,初始pH 7.04,培养温度30℃,摇床转速169 r/min的条件下,亚硝氮和总氮去除率分别达到99.14%和97.06%,优于椭圆小球藻单藻和反硝化细菌TC-1单菌的的除氮效果,显示了其在废水脱氮中具有良好的应用前景。In order to improve the nitrogen removal efficiency of aquaculture wastewater, a species of aerobic denitrifying bacterium TC-1 was isolated from the water of intensive aquaculture pond. The denitrifying bacterium TC-1 was identified by means of morphological observation, physiological and biochemical tests and 16 S rDNA sequence analysis. In addition,Chlorella ellipsoides was selected, which together with denitrifying bacteria TC-1 as the research objectives, the effects of carbon sources, inoculation amount, initial pH value, culture temperature and shaking table speed on nitrogen removal performance of the system were studied by bacteria algae co-culture method. Besides, a three-factors and three-levels Box-Behnken experimental design and response surface methodology were used to optimize the culture conditions. The results showed that strain TC-1 was identified as Pseudomonas toyotomiensis TC-1;and the optimal nitrogen removal condition was sodium succinate used as a carbon source, total inoculum volume 2%, initial pH 7.04, culture temperature:30 ℃;and rotation speed: 169 r/min. Under the optimum condition, removal rates of nitrite and total nitrogen reached 99.14%and 97.06%, respectively, which suggested that this bacteria-and-algae co-culture system was a prospective technology used in denitrification of wastewater.

关 键 词：好氧反硝化细菌 菌藻共培养 椭圆小球藻 除氮特性 

分 类 号：X172[环境科学与工程—环境科学]