牙龈卟啉单胞菌脂多糖促进食管鳞癌细胞的增殖和迁移  

Porphyromonas gingivalis lipopolysaccharide promotes the proliferation and migration of esophageal squamous cell carcinoma cells

作　　者：张庆慧 刘晓波 文卉 李胜保 童强 ZHANG Qinghui;LIU Xiaobo;WEN Hui;LI Shengbao;TONG Qiang(Department of Gastroenterology,Guangzhou Red Cross Hospital Affiliated to Jinan University,Guangdong Guangzhou 510220,China;Department of Gastroenterology,Taihe Hospital,Affiliated Hospital of Hubei University of Medicine,Hubei Shiyan 442000,China)

机构地区：[1]暨南大学附属广州红十字会医院消化内科,广东广州510220 [2]十堰市太和医院,湖北医药学院附属医院消化内科,湖北十堰442000

出　　处：《现代肿瘤医学》2021年第23期4122-4128,共7页Journal of Modern Oncology

基　　金：湖北省卫生健康科研基金(编号:WJ2021M046);湖北省十堰市科技局项目(编号:21Y19);湖北医药学院自由探索基金项目(编号:FDFR201904);十堰市太和医院院级科研基金(编号:2019JJXM032,2020JJXM032)。

摘　　要：目的:评估牙龈卟啉单胞菌脂多糖(Pg-LPS)对食管鳞癌细胞增殖、迁移能力的影响,探索其可能的机制。方法:培养Het-1A和EC109细胞,实验组加入不同浓度Pg-LPS,对照组加入等量培养基。分别采用CCK-8、Transwell迁移实验或细胞划痕实验检测两组细胞增殖、迁移能力的变化;qRT-PCR、Western blot技术检测增殖、迁移相关基因表达量的改变。结果:Het-1A细胞经Pg-LPS作用24 h、48 h后,其光密度值较对照组显著上升。EC109细胞在5μg/mL及10μg/mL的Pg-LPS作用后24 h、48 h与对照组相比光密度值上升;1μg/mL的Pg-LPS作用24 h后光密度值较对照组上升,差异有统计学意义(P<0.05),而作用48 h后,无明显统计学差异(P>0.05)。Pg-LPS作用后,Het-1A细胞穿膜数量多于对照组,EC109细胞相对覆盖面积高于对照组。Pg-LPS作用后Het-1A和EC109细胞增殖、迁移相关基因mRNA及ARTN蛋白表达量较对照组明显升高。结论:Pg-LPS可以促进Het-1A和EC109细胞的增殖、迁移;Pg-LPS作用于细胞后ARTN的表达量增加,进一步促进AKT1、CCND1、MTA1、CXCR4表达,可能是Pg-LPS影响食管鳞癌细胞增殖和迁移的机制之一。Objective:To evaluate the effect of porphyromonas gingivalis lipopolysaccharide(Pg-LPS)on the proliferation and migration of esophageal squamous cell carcinoma cells,and explore its possible mechanisms.Methods:The Het-1A and EC109 cells were cultured in vitro.In the experimental group,Het-1A and EC109 cells were cultured in the presence of Pg-LPS,while Het-1A and EC109 cells in the control group were treated with the same amount of medium.The proliferation and migration of Het-1A and EC109 cells in the control group or Pg-LPS treated group were detected by CCK-8,Transwell or wound healing assays.qRT-PCR and Western blot were used to evaluate the changes of gene expression levels related to proliferation and migration.Results:The optical density values of Het-1A cells treated with Pg-LPS for 24 or 48 hours were significantly higher than that of the control group.The optical density values of EC109 cells after treated with 5μg/mL or 10μg/mL Pg-LPS for 24 or 48 hours were significantly higher than that of the control group.After treatment with 1μg/mL Pg-LPS for 24 h,the optical density values of EC109 cells increased compared with the control group,the difference was statistically significant(P<0.05),but there was no significant difference after 48 hours(P>0.05).The number of Het-1A cells penetrating membrane was significantly higher than that of the control group after treated with Pg-LPS.The relative coverage area of EC109 cells was significantly higher than that of the control group after treated with Pg-LPS for 24 hours or 48 hours.The mRNA expression of genes related to proliferation and migration and the expression level of ARTN protein in Het-1A and EC109 cells treated with Pg-LPS were significantly higher than those in the control group.Conclusion:Pg-LPS could promote the proliferation and migration of Het-1A and EC109 cells.The expression level of ARTN increased after treated with Pg-LPS,and further promoted the expression of AKT1,CCND1,MTA1 and CXCR4,which may be one of the mechanisms of Pg-LPS affectin

关 键 词：牙龈卟啉单胞菌 脂多糖 食管上皮细胞 食管鳞癌细胞 增殖 迁移 

分 类 号：R735.1[医药卫生—肿瘤]