MicroRNA-218单核苷酸多态性与结直肠癌易感性的关系  

Association between microRNA-218 single nucleotide polymorphism and the susceptibility to colorectal cancer

作　　者：李天煜 庞彩莲 徐瑞梅 林悠阅 吕昕琪 范志豪 钟展华 何俊力 袁朝汉 魏驰誉 黄舒琪 谢烷颐 廖日煜 LI Tian-yu;PANG Cai-lian;XU Rui-mei;LIN You-yue;LYU Xin-qi;FAN Zhi-hao;ZHONG Zhan-hua;HE Jun-li;YUAN Chao-han;WEI Chi-yu;HUANG Shu-qi;XIE Wan-yi;LIAO Ri-yu(Department of Surgery,Guangdong Medical University,Dongguan 523110,Guangdong,China;不详)

机构地区：[1]广东医科大学外科学教研室,广东东莞523808 [2]东莞市大朗医院检验科,广东东莞523770 [3]广东医科大学公共卫生学院,广东东莞523808 [4]广东医科大学研究生学院,广东东莞523808

出　　处：《广东医学》2021年第11期1301-1306,共6页Guangdong Medical Journal

基　　金：东莞市社会科技发展(重点)项目(2017507152461);广东医科大学大学生创新实验项目(FZDS001);广东医科大学校级大学生创新创业训练计划项目(GDMU2017036,GDMU2019022,GDMU2019073);广东省大学生创新创业训练计划项目(S202010571073);广东省国家级大学生创新创业训练计划项目(201810571036,202010571022)。

摘　　要：目的探究microRNA-218单核苷酸多态性与结直肠癌易感性的关联。方法应用聚合酶链式反应-连接酶检测反应技术(PCR-LDR)对66例结直肠癌患者和124例健康对照者的microRNA-218单核苷酸的多态性进行分析,并采用二元逻辑回归分析评估此多态位点与结直肠癌易感性的相关性。同期对比检测microRNA-25、microRNA-143和microRNA-106a的单核苷酸多态性。结果二元逻辑回归分析结果显示,相比AG和AA基因型,microRNA-218 rs11134527的GG基因型显著增加结直肠癌的易感性(OR=2.31,P<0.05)。而microRNA-25 rs41274221、microRNA-143 rs4705342和microRNA-106a rs137881717的单核苷酸多态性与结直肠癌的易感性无关联(P>0.05)。结论MicroRNA-218 rs11134527单核苷酸多态性与结直肠癌易感性密切相关。Objective To investigate the function of microRNA-218 in colorectal cancer(CRC).Methods The polymerase chain reaction-ligation detection reaction(PCR-LDR)method was used to genotype the polymorphism of microRNA-218 in 66 CRC cases and 124 healthy controls.Furthermore,association between the risk for CRC and the four SNPs was evaluated via binary logistic regression analysis.Results Binary logistic regression analysis showed that GG genotype(OR=2.31,P<0.05)of microRNA-218 rs11134527 was significantly correlated to the increased risk of CRC,compared with the AG and AA genotype.However,no significant association was found between the SNP rs41274221 in microRNA-25(P>0.05),the SNP rs4705342 in microRNA-143(P>0.05)or the SNP rs137881717 in microRNA-106a(P>0.05)with CRC.Conclusion Micro218 rs11134527 is closely correlated to the susceptibility to CRC.

关 键 词：microRNA-218 单核苷酸多态性 结直肠癌 易感性 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]