基于高通量测序的水貂胚胎滞育期和激活期卵巢转录组分析  

Transcriptomic Analysis of Mink Ovaries at Embryonic Diapause and Activation Stages Based on High-throughput Sequencing Technology

作　　者：韩玉萍 赵向远 范冰峰 刘理想 邵静 许保增[1] HAN Yuping;ZHAO Xiangyuan;FAN Bingfeng;LIU Lixiang;SHAO Jing;XU Baozeng(Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112, China)

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《中国畜牧兽医》2021年第11期4035-4046,共12页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省实验动物领域创新研究项目(20210506022ZP);吉林省自然科学基金(20190201170JC);吉林省国际科技合作项目(20170414049GH);中国农业科学院科技创新工程项目(CAAS-ASTIP-2020-ISAPS)。

摘　　要：试验旨在获取胚胎滞育期与激活期水貂卵巢组织的转录组信息,挖掘滞育的胚胎激活前后水貂卵巢差异表达基因(DEGs)及其功能信息,为探讨卵巢信号调控水貂胚胎滞育的分子机制提供参考。随机采集8只健康雌性水貂(胚胎滞育期和激活期各4只)的卵巢组织为样本,利用Illumina HiSeq平台RNA-Seq技术对其进行转录组测序,筛选在水貂胚胎滞育期和激活期卵巢中的DEGs并进行生物信息学分析。结果显示,测序获得661195568个raw reads,经过滤后获得650834900个clean reads,组装后得到389895个unigenes,通过与Nr、GO、KOG和KEGG数据库比对,对unigenes进行注释,其中Nr数据库注释了156419个unigenes;GO数据库注释了122657个unigenes;KOG数据库注释了58320个unigenes;KEGG数据库注释了72653个unigenes。滞育期和激活期卵巢中有1797个DEGs,与胚胎滞育期水貂卵巢相比,激活期卵巢有1298个DEGs显著上调,499个DEGs显著下调。GO功能分析发现,DEGs显著富集的生物学过程主要有跨膜信号受体活性、信号受体活性、G蛋白偶联受体活性、酶联受体蛋白信号通路、细胞表面受体信号通路、细胞周期阻滞、芳香酯酶活性。KEGG通路分析发现,水貂滞育期和激活期卵巢中的DEGs显著富集于神经活动配体-受体相互作用信号通路。本研究利用高通量测序技术获得水貂胚胎滞育期与胚胎激活期卵巢的转录组信息,为深入探究水貂卵巢调节胚胎滞育的分子机制奠定了基础。This experiment was aimed to obtain the mink ovarian transcriptome at the embryonic diapause and activation stages,dig the differentially expressed genes(DEGs)between them,and explore the biological functions of DEGs,which could provide a reference for investigating the molecular mechanisms underlying ovarian signals regulate the embryonic diapause and activation.Ovaries were collected randomly from eight healthy female mink(four mink for embryonic diapause and activation stages)were processed for transcriptomic sequencing using Illumina HiSeq platform RNA-Seq technology.Ovarian DEGs were screened and analyzed through bioinformatics.The results showed that a total of 661195568 raw reads were obtained by sequencing,650834900 clean reads were obtained by filtering,and 389895 unigenes were obtained by assembly.unigenes was annotated by comparing with Nr,GO,KOG and KEGG databases.156419,122657,58320 and 72653 unigenes were annotated in Nr,GO,KOG and KEGG databases,respectively.There were 1797 DEGs in mink ovaries at the embryonic diapause and activation stages.Compared with the embryonic diapause mink ovary,1298 DEGs in the activation stage ovaries were significantly up-regulated and 499 DEGs were significantly down-regulated.GO function analysis showed that the biological processes of DEGs significantly enriched mainly included transmembrane signaling receptor activity,signaling receptor activity,G-protein coupled receptor activity,enzyme linked receptor protein signaling pathway,cell surface receptor signaling pathway,cell cycle arrest,arylesterase activity.KEGG pathway analysis showed that the DEGs in the mink ovaries during embryonic diapause and activation stages were significantly enriched in the neuroactive ligand-receptor interaction.In this study,high-throughput sequencing technology was used to obtain transcriptome information of mink ovaries during embryonic diapause stage and during embryonic activation stage,which laid a foundation for further investigating the roles of mink ovary in regulating embryonic

关 键 词：水貂 卵巢 胚胎滞育期 胚胎激活期 转录组 RNA-SEQ 

分 类 号：S814.8[农业科学—畜牧学]