基于TCGA数据集分析CSTF2基因在肝细胞癌中的表达和临床意义  

Expression and clinical significance of CSTF2 gene in hepatocellular carcinoma based on TCGA dataset

作　　者：王雪[1] 郭传影 王侠 曹生亚[1] Wang Xue;Guo Chuanying;Wang Xia;Cao Shengya(Department of Laboratory Medicine,Xuzhou Tumor Hospital,Xuzhou 221005,China)

机构地区：[1]徐州市肿瘤医院检验科,江苏徐州221005

出　　处：《实用肿瘤杂志》2021年第6期550-555,共6页Journal of Practical Oncology

摘　　要：目的研究裂解刺激因子亚单位2(cleavage stimulation factor subunit 2,CSTF2)基因在肝细胞癌中的表达情况以及其表达与患者临床特征和预后的关系,探究CSTF2基因在肝细胞癌中作用的分子机制。方法从癌症基因图谱(The Cancer Genome Atlas,TCGA)官方数据库下载肝细胞癌原始RNA测序数据和患者的临床资料,利用R 4.0.2软件提取CSTF2基因表达值,分析其在肝细胞癌组织表达情况,用χ2检验分析CSTF2基因表达与患者临床特征的关系,用Kaplan-Meier法分析CSTF2表达与患者预后的关系,用Cox风险比例模型分析CSTF2的预后价值。基因集富集分析(gene set enrichment analysis,GSEA)用于探究CSTF2基因在肝细胞癌中的分子机制。结果与癌旁组织比较,肝细胞癌组织中CSTF2表达水平升高(P<0.01),CSTF2高表达组和低表达组患者在TNM分期(P=0.024)和T分期(P=0.008)方面比较,差异均具有统计学意义。CSTF2高表达组患者总体生存率低于低表达组(P<0.01)。Cox回归分析显示,CSTF2表达为肝细胞癌患者的独立预后因素(HR=1.107,95%CI:1.045~1.172,P=0.001)。GSEA分析结果显示,CSTF2参与基因转录因子、细胞周期、同源重组、DNA降解和哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号途径等分子机制。结论CSTF2基因在肝细胞癌中表达上调,并与患者的不良预后相关,为肝细胞癌患者独立预后因素,有望成为肝细胞癌潜在分子标志物。Objective To analyze the expression of cleavage stimulation factor subunit 2(CSTF2)gene in hepatocellular carcinoma(HCC),and to explore its clinical significance,prognostic value and molecular mechanism in HCC.Methods The original RNA sequencing data of HCC and the corresponding clinical data of patients were downloaded from the official database of The Cancer Genome Atlas(TCGA).R 4.0.2 software was utilized to extract the expression value of CSTF2 gene.χ2 test was used to analyze the relationship between CSTF2 gene expression and patients’clinical characteristics,and the Kaplan-Meier method was used to analyze the relationship between CSTF2 expression and the prognosis of the patients.Cox risk ratio model was used to analyze the prognostic value of CSTF2.Gene Set Enrichment Analysis(GSEA)analysis was used to explore the molecular mechanism of CSTF2 gene in HCC.Results Compared with adjacent tissues,the expression level of CSTF2 gene in HCC tissues was significantly increased(P<0.01),and there were statistically significant differences in TNM stage(P=0.024)and T stage(P=0.008)between patients with high and low CSTF2 expression.Survival analysis showed that the overall survival rate of patients with high CSTF2 expression was significantly lower than that of patients with low CSTF2 expression(P<0.01).Cox regression analysis showed that CSTF2 expression was an independent prognostic factor for patients with HCC(HR=1.107,95%CI:1.045-1.172,P=0.001).GSEA analysis results showed that CSTF2 was involved in molecular mechanisms such as gene transcription factors,cell cycle,homologous recombination,DNA degradation,and mammalian target of rapamycin(mTOR)signaling pathway.Conclusions The expression of CSTF2 gene is significantly up-regulated in HCC and is related to the poor prognosis of patients.CSTF2 gene is an independent prognostic factor of HCC patients,and is a potential molecular marker of HCC.

关 键 词：肝细胞癌 CSTF2 预后 

分 类 号：R735.7[医药卫生—肿瘤]