水稻抗白叶枯病基因Xa23分子标记的开发与应用  

Development and Utilization of Bacterial Blight Resistance Gene Xa23 Molecular Marker

作　　者：王闵霞[1] 白玉路[1] 张琼 徐登武 张志勇[1] 王平[1] WANG Min-xia;BAI Yu-lu;ZHANG Qiong;XU Deng-wu;ZHANG Zhi-yong;WANG Ping(Institute of Biotechnology and Nuclear Technology,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China)

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066

出　　处：《西南农业学报》2021年第10期2070-2075,共6页Southwest China Journal of Agricultural Sciences

基　　金：四川省科技厅应用基础项目(2018JY0176);现代农业学科建设推进工程(2021XKJS055);四川省科技计划重点研发项目(2021YFYZ0016)。

摘　　要：【目的】白叶枯病是水稻主要病害之一,经常会引起水稻严重减产。利用寄主自身对白叶枯病的抗性,不仅能提高水稻产量,还能减少因大量施用农药造成的环境污染。研究表明Xa23对白叶枯病有较好抗性并已成功用于水稻抗白叶枯病抗性育种中。已有的Xa23分子标记均位于基因外侧。为了提高分子标记的准确性,并简化分子标记辅助选择步骤,本研究拟开发一个位于Xa23基因内部的分子标记以利于水稻抗白叶枯病育种实践。【方法】抗白叶枯病材料CBB23与感病材料JG30、日本晴、MDJ8等在Xa23基因启动子区存在序列差异和缺失,通过测序确认该差异序列存在于待改良材料川恢907、川航恢908、川恢991、川恢992中,针对差异序列设计引物开发分子标记,该引物以Xa23基因供体CBB23的DNA为模板能扩增出特异PCR产物,其他则不能扩增到特异PCR产物。利用该分子标记对部分水稻重要种质资源的Xa23基因型进行了鉴定。【结果】本研究开发了一个位于Xa23基因启动子区的分子内标记,该分子标记为显性标记,以阳性或杂合体对照DNA为模板均扩增到一条198 bp左右的亮带,阴性对照中则无对应的条带。利用该标记对354个引自不同稻区的水稻亲本进行Xa23基因背景鉴定,发现5个材料包括华航G528、R418、R419、R433、R440含有Xa23基因,其他亲本不含有Xa23基因。【结论】本研究开发了一个位于Xa23基因内部的分子标记,这不仅提高了分子标记辅助育种的准确性,还通过简化检测步骤提升了检测效率。本标记的开发可为Xa23的高效利用提供技术参考。【Objective】Bacterial Blight disease is one of the main diseases of rice,which often causes severe rice yield reduction.Using the resistance of host to bacterial blight disease can not only improve rice yield,but also reduce the environmental pollution caused by the massive application of pesticides.The results showed that Xa23 has good resistance to bacterial blight disease and has been successfully used in rice breeding for bacterial blight resistance.The developed Xa23 molecular markers are located on the lateral side of the gene.In order to improve the accuracy of molecular markers and simplify the process of marker-assisted selection,we intend to develop a molecular marker located in the Xa23 gene to facilitate the rice breeding for better bacterial blight resistance.【Method】There were sequence differences and deletion in Xa23 promoter region between Bacterial Blight resistant rice CBB23 and susceptible rice JG30,Nipponbare,MDJ8,etc.The differential sequence was confirmed to exist in chuanhui907,Chuanhanghui908,Chuanhui991,and Chuanhui992 by sequencing.Primers were designed to develop molecular markers based on the differential sequences.The primers could amplify specific PCR products using the DNA of Xa23 gene donor CBB23 as template,but could not amplify specific PCR products from others.This molecular marker was used to identify Xa23 genotypes of some important rice germplasm.【Result】In the study,we developed a molecular marker located in the promoter region of Xa23 gene,which was dominant and could amplify specific 198 bp PCR product when using positive or heterozygous reference DNA as template,while there was no corresponding PCR product in negative.The Xa23 genotypes of 354 important rice germplasm from different rice regions was identified by using this marker.It was found that 5 rice germplasm,including HuahangG528,R418,R419,R433 and R440,contained the Xa23 gene,but others did not contain.【Conclusion】In this study,a molecular marker located in Xa23 gene was developed,which not only imp

关 键 词：白叶枯病 XA23 分子标记 显性 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]