Altered metabolic state impedes limb regeneration in salamanders  

作　　者：Zhe-Lun Peng Bin-Xu Yin Rui-Min Ren Yin-Long Liao Hao Cai Heng Wang 

机构地区：[1]College of Animal Sciences and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China [2]Shenzhen Institute of Nutrition and Health,Huazhong Agricultural University,Shenzhen,Guangdong 518120,China [3]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China

出　　处：《Zoological Research》2021年第6期772-782,共11页动物学研究（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(31771617)and HZAU-AGIS Cooperation Fund(SZYJY2021009)。

摘　　要：Salamanders are unique among tetrapods in their ability to regenerate the limbs throughout life.Like other poikilothermic amphibians,salamanders also show a remarkable capacity to survive long periods of starvation.Whether the physiological reserves necessary for tissue regeneration are preserved or sacrificed in starved salamanders is unknown.In the current study,we maintained Iberian ribbed newts(Pleurodeles waltl)under extreme physiological stress to assess the extent of regeneration and identify the molecular and cellular changes that may occur under such conditions.After 19 months of complete food deprivation,the animals exhibited extensive morphological and physiological adaptations but remained behaviorally active and vigilant.Autophagy was elevated in different tissues and the transformed gut microbiota indicated remodeling of the intestinal tract related to autophagy.Upon limb amputation in animals starved for 21 months,regeneration proceeded with progenitor cell proliferation and migration,leading to limb blastema formation.However,limb outgrowth and patterning were substantially attenuated.Blockage of autophagy inhibited cell proliferation and blastema formation in starved animals,but not in fed animals.Hence,tissue autophagy and the regenerative response were tightly coupled only when animals were under stress.Our results demonstrate that under adverse conditions,salamanders can exploit alternative strategies to secure blastema formation for limb regeneration.

关 键 词：STARVATION Stress AUTOPHAGY Regeneration SALAMANDER 

分 类 号：Q418[生物学—生理学]