细胞松弛素B阻断微核法鉴定男性个体辐射敏感性的方法初探  

Preliminary Study on Male Individual Radiosensitivity by Cytochalasin-B Blocked Micronucleus Assay

作　　者：肖昌浩 王萍[2] 冯骏超 郑利军 黄皓 潘书贤 徐嘉宁 靳淑红 李冰燕[2] 周光明[1] 聂晶[1] XIAO Changhao;WANG Ping;FENG Junchao;ZHENG Lijun;HUANG Hao;PAN Shuxian;XU Jianing;JIN Shuhong;LI Bingyan;ZHOU Guangming;NIE Jing(State Key Laboratory of Radiation Medicine and Protection,School of Radiation Medicine and Protection,Soochow University,Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions,Suzhou 215000,Jiangsu,China;School of Public Health,Soochow University,Suzhou 215123,Jiangsu,China;Department of Nuclear Accident Medical Emergency,Second Affiliated Hospital of Soochow University,Suzhou 215004,Jiangsu,China)

机构地区：[1]省部共建放射医学与辐射防护国家重点实验室,苏州大学医学部放射医学与防护学院,江苏省高校放射医学协同创新中心,江苏苏州215000 [2]苏州大学医学部公共卫生学院,江苏苏州215123 [3]苏州大学附属第二医院核事故医学应急办公室,江苏苏州215004

出　　处：《原子核物理评论》2021年第3期339-344,共6页Nuclear Physics Review

基　　金：国家重点研发计划项目(2018YFC0115704)。

摘　　要：初步研究了男性个体辐射敏感性的鉴定方法及标准。采集50名男性志愿者的外周血,分别给予不同剂量X射线照射,采用细胞松弛素B阻断双核法测定微核率(MNF),通过二阶多项拟合法,绘制微核剂量效应选项中心标准曲线,将个人微核剂量效应曲线与标准曲线比对后判断个体辐射敏感性。0.0∼2.5 Gy剂量范围内,剂量效应二阶多项拟合的中心方程为(MNF=0.0147+0.0362D+0.0231D^(2),r=0.726)。50名志愿者中,辐射敏感的有13人,辐射抗性的有14人,基本符合正态分布。Spearman秩和相关分析结果显示,MNF在各个辐射剂量点与辐射敏感性均存在正相关,与辐射抗性呈负相关,MNF随剂量增加而增加。本研究初步建立了“以线代点”男性个体辐射敏感性鉴定方法,并发现男性外周血淋巴细胞的本底微核率与个体辐射敏感性呈正相关。Methods and criteria for the identification of male individual radiosensitivity are investigated.The peripheral blood of 50 male volunteers was irradiated with different doses of X-ray,the micronucleus frequency(MNF)was measured by cytokinesis-B block micronucleus assay,and the central standard curve of micronucleus dose effect was drawn by second-order multinomial fitting method so that the individual dose-response curve of micronucleus was compared with the standard curve to judge individual radiosensitivity.In the dose range of 0.0~2.5 Gy,the central equation of second-order polynomial fit for dose effect is(MNF=0.0147+0.0362D+0.0231D^(2),R=0.726).Among the 50 male volunteers,13 were sensitive to radiation(26.0%)and 14 were resistant(28.0%),which basically fits the normal distribution.Spearman rank correlation analysis showed that the MNF increased with the dose and there was a positive correlation between the background MNF and the radiosensitivity of each dose,but negative correlation with the radioresistance.In conclusion,a"curve-instead-of-dose"male individual radiosensitivity identification method was established.Moreover,it was found that the background of micronuclei in peripheral blood lymphocytes was positively correlated with individual radiosensitivity.

关 键 词：男性个体辐射敏感性 电离辐射 微核 剂量依赖性 

分 类 号：R735.7[医药卫生—肿瘤]