长期中小强度有氧运动对大鼠左心室肌蛋白质组差异表达的影响  

Effects of long-term moderate-small intensity aerobic exercise on differential expression of proteome in left ventricular muscle of rats

作　　者：郭远盘 史绍蓉[2] 徐哲 王娟[4] 黄思敏[5] 万莉莉 雷雄[6] 彭勇[7] GUO Yuan-pan;SHI Shao-rong;XU Zhe;WANG Juan;HUANG Si-min;WAN Li-li;LEI Xiong;PENG Yong(P.E.Dept.,Changsha Normal University,Changsha 410010;P.E.Dept.,Hunan Normal University,Changsha 410012;P.E.Dept.,Hunan City University,Yiyang 413039;Beijing Sport University,Beijing 100084;Sports and Health Department,Zhaoqing University,Zhaoqing 526061;Xiangya Hospital,Central South University,Changsha 410008;Nanjing Sport Institute,Nanjing 210014,China)

机构地区：[1]长沙师范学院体育科学学院,湖南长沙410010 [2]湖南师范大学体育学院,湖南长沙410012 [3]湖南城市学院体育学院,益阳413039 [4]北京体育大学,海淀100084 [5]肇庆学院体育与健康学院,广东肇庆526061 [6]中南大学湘雅医院,湖南长沙410008 [7]南京体育学院运动健康科学系,江苏南京210014

出　　处：《中国应用生理学杂志》2021年第6期673-677,698,共6页Chinese Journal of Applied Physiology

基　　金：湖南省科技厅计划项目(2007FJ3075);湖南省教育厅科学研究项目(19C0145)。

摘　　要：目的:探讨长期中小强度有氧运动对大鼠左室肌蛋白质组差异性表达的影响,筛选出对中小强度有氧运动刺激敏感的目标蛋白质,丰富运动健身的基础理论及为慢性心血管疾病的康复治疗提供新的思路和实验依据。方法:20只雄性SD大鼠随机分为运动组(E组)和对照组(C组)(n=10)。建立大鼠长期中小强度有氧运动跑台训练模型,采用双向凝胶电泳(2-DE)对大鼠左心室肌全蛋白样品进行分离。采用串联飞行时间质谱蛋白质仪对部分分离后差异表达量上调大于5倍或下调超过80%的蛋白质点进行质谱鉴定。结果:与C组比较,E组大鼠心脏重量指数(HWI)增加了32.0%,具有显著性差异(P<0.05);与C组比较,E组有71个蛋白质点表达上调≥2倍或下调≥50%,对4个表达上调≥5倍或下调大于等于80%的蛋白质点进行质谱鉴定,鉴定出3个蛋白质和1个未知蛋白质。结论:长期中小强度有氧运动后,大鼠心脏发生了良好的适应性改变;大鼠左心室肌蛋白质组发生了明显变化,长期中小强度有氧运动能有效增强大鼠心肌抗氧化能力。Objective:To investigate the effects of long-term moderate-small intensity aerobic exercise on the differential expression of proteome in left ventricular muscle of rats,and to screen the target proteins sensitive to moderate-small intensity aerobic exercise stimulation.This study will enrich the basic theory of exercise and fitness and provide new ideas and experimental basis for the rehabilitation treatment of chronic cardiovascular disease.Methods:Twenty male SD rats were randomly divided into exercise group and control group(n=10).The treadmill training model of long-term moderate-small intensity aerobic exercise was established,and the whole protein samples of left ventricular muscle were extracted and separated by two-dimensional gel electrophoresis(2-DE).The two-dimensional gel electrophoresis map was analyzed by Bio PD quest image analysis software.The protein spots with differential expression more than 5 times or down-regulated over 80%after exercise were identified by tandem time-of-flight mass spectrometry(ULGRAFL-FLEX-TOF/TOF).Results:Compared with the group C,the heart weight index of the group E was increased by 32.0%,and the difference was significant(P<0.05).Compared with the group C,there were 71 protein spots expression were up-regulated≥2 times or down-regulated≥50%in the group E.4 protein spots expression were up-regulated≥5 times or down-regulated≥80%were identified by mass spectrometry,3 proteins and 1 unknown protein were identified.Conclusion:After long-term moderate-small intensity aerobic exercise,the rats heart had a good adaptive change,and the proteome of left ventricular muscle changed significantly.Long-term moderate-small intensity aerobic exercise can effective enhance the ability of myocardial antioxidation.

关 键 词：蛋白质组 左心室肌 中小强度有氧运动 双向凝胶电泳 质谱鉴定 

分 类 号：G804[文化科学—运动人体科学]