负载羟固醇小牛骨块对犬颌骨缺损修复早期的影响  

Impact of oxysterols coated bovine bone blocks for regeneration of critical-sized defects in dog mandible at the early stage of bone healing

作　　者：胡晓 刘威 吴王喜 周磊 Hu Xiao;Liu Wei;Wu Wangxi;Zhou Lei(Stomatology Hospital,School of Stomatology,Zhejiang University School of Medicine,Clinical Research Center for Oral Diseases of Zhejiang Province,Key Laboratory of Oral Biomedical Research of Zhejiang Province,Cancer Center of Zhejiang University,Hangzhou 310006,China;School Stomatology Zhengzhou University,The first affiliated hospital Zhengzhou University,Zhengzhou 450000,China;Stomatological Hospital of Southern Medical University,Guangzhou 510280,China;Taikang Bybo Dental Group,Guangzhou 510280,China)

机构地区：[1]浙江大学医学院附属口腔医院浙江大学口腔医学院浙江省口腔疾病临床医学研究中心浙江省口腔生物医学研究重点实验室浙江大学癌症研究院,杭州310006 [2]郑州大学口腔医学院郑州大学第一附属医院,郑州450000 [3]南方医科大学口腔医院,广州510280 [4]泰康拜博口腔,广州510280

出　　处：《中国口腔种植学杂志》2021年第5期289-293,共5页Chinese Journal of Oral Implantology

基　　金：国家自然科学基金(81170998)。

摘　　要：目的探究负载羟固醇小牛骨块(羟固醇骨块)对比格犬下颌骨标准骨缺损修复早期的影响。方法选取雄性比格犬4只,拔除下颌骨双侧第三、第四前磨牙及第一磨牙后,在左、右两侧下颌骨颊侧各制备2个5 mm×10 mm×10 mm箱型标准骨缺损(n=4/只,n=16)。愈合4周后,按双侧同名区域对照设计,随机将羟固醇骨块(n=8)植入一侧骨缺损,小牛骨块(n=8)植入另一侧骨缺损。4周后处死,对获取标本处理后制作石蜡切片,进行组织学观察和形态学测量。结果羟固醇骨块组和小牛骨块组均观察到骨缺损内部有明显新骨形成。HE染色组织形态学测量结果示羟固醇骨块组的新生骨面积百分比为(35.43±2.78)%,高于小牛骨块组(30.88±2.03)%(P<0.05);羟固醇骨块组新生血管数量为(31.50±1.60)个,小牛骨块组为(30.00±1.31)个,差异无统计学显著性(P>0.05)。骨钙素免疫组织化学染色结果示羟固醇骨块组骨钙素阳性细胞数为(63.50±7.50)个,高于小牛骨块组(55.50±5.15)个(P<0.05)。结论羟固醇骨块促进犬下颌骨标准骨缺损的再生修复与羟固醇能促进骨愈合早期阶段的成骨细胞的分化成熟有关。Objective To explore the impact of oxysterols coated bovine bone blocks(OBB)for regeneration of critical-sized defects in dog mandible at the early stage of bone healing.Methods Four beagle dogs were used in this study.After the mandibular third,fourth premolar,and first molar extraction,two standardized box-shaped defects(5 mm×10 mm×10 mm)were created on both sides of the mandible of each animal(n=4 animals,total 16 defects).Four weeks later,the defects were randomly augmented with OBB(n=8)or bovine bone blocks(n=8)in a split-mouth design.All animals were sacrificed 4 weeks after surgery.The specimens were decalcified for hematoxylin and eosin staining and immunohistochemical analysis of osteocalcin(OC).Results Histomorphometrical analysis(HE staining)revealed that the percentage of new bone was significantly higher in the OBB group(35.43±2.78)%in comparison with the BBB group(30.88±2.03)%,P<0.05.And the mean value of the number of vessels was(31.50±1.60)in the OBB group and(30.00±1.31)in the BBB group(P>0.05).The number of OC-stained cells was(63.50±7.50)in the OBB group and 55.50±5.15 in the BBB group.There is a significant difference in the numbers of OC-stained cells(P<0.05).Conclusion OBB can enhance the regeneration of critical-sized defects by stimulating the differentiation of osteoblastic cells at the early stage of bone healing.

关 键 词：羟固醇 成骨细胞 骨缺损 

分 类 号：R782.4[医药卫生—口腔医学]