消化道肿瘤患者外周血T淋巴细胞亚群及血清VEGF水平联合检测的临床意义  

Clinical Significance of Detection of Peripheral Blood T Lymphocyte Subsets and Serum VEGF Levels in Patients with Gastrointestinal Tumors

作　　者：沙敏[1] 栾正云[1] SHA Min;LUAN Zheng-yun(Taizhou People's Hospital,Taizhou Jiangsu 225300,China)

机构地区：[1]泰州市人民医院中心实验室,江苏泰州225300

出　　处：《泰州职业技术学院学报》2021年第5期77-80,共4页Journal of Taizhou Polytechnic College

基　　金：2019年泰州市人民医院院级课题(ZL201930,课题负责人:沙敏);泰州市医学创新团队(CXTDB201904,团队主持人:于鸿).

摘　　要：目的通过分析消化道肿瘤患者外周血T淋巴细胞亚群、血清血管内皮生长因子(VEGF)水平,探讨两者联合检测在消化道肿瘤患者临床诊断中的应用价值。方法分别应用流式细胞术和酶联免疫吸附法(ELISA)检测146例消化道肿瘤患者及35名健康对照组外周血T淋巴细胞亚群、血清VEGF水平,并分析两者水平与消化道肿瘤患者临床生物学行为的相关性。结果消化道肿瘤患者CD4+和CD3+T细胞亚群以及CD4+/CD8+比值明显低于对照组(P<0.05),而CD8+T细胞亚群和血清VEGF水平明显高于对照组(P<0.05)。消化道肿瘤患者CD4+、CD8+和CD3+T细胞亚群、CD4+/CD8+比值以及血清VEGF水平与肿瘤患者的临床TNM分期和病理分级密切相关(P<0.05)。结论通过联合检测消化道肿瘤患者外周血T淋巴细胞亚群和血清VEGF水平,为判断肿瘤发生、生长转移等过程以及免疫辅助治疗提供可能的依据。Objective To investigate the clinical significance of peripheral blood T lymphocyte subsets and serum VEGF levels in patients with gastrointestinal tumors.Methods To detect peripheral blood T lymphocyte subsets and serum VEGF levels in 146 patients with gastrointestinal tumors and 35 healthy controls by Flow cytometry and enzyme-linked immunosorbent assay(ELISA)and study the correlation with clinical biological behavior of gastrointestinal tumors.Results The CD4+and CD3+T cell subsets and the CD4+/CD8+ratio in patients with gastrointestinal malignancies were significantly lower than those in the control group(P<0.05),while the CD8+T cell subsets and the level of serum VEGF were significantly higher than the control group(P<0.05).The CD4+/CD3+T cell subsets/the CD4+/CD8+ratio and the level of serum VEGF was positively correlated with clinical stage and the pathological grade(P<0.05).Conclusion The combined detection of peripheral blood T lymphocyte subsets and serum VEGF levels in patients with gastrointestinal tumors provides a possible basis for judging tumor growth status and immune adjuvant therapy.

关 键 词：消化道肿瘤 T淋巴细胞亚群 血管内皮生长因子 

分 类 号：R735[医药卫生—肿瘤]