基于功能性近红外光谱技术的儿童与成人视皮层区功能活动研究  

Study on functional activities of visual cortex in children and adults by functional near infrared spectroscopy

作　　者：张颖[1] 曹宁 毕爱玲[2,3] 吴晓彤 张莉唯 龚胜 吴建峰 温莹[4] 毕宏生[2,3,4] ZHANG Ying;CAO Ning;BI Ailing;WU Xiaotong;ZHANG Liwei;GONG Sheng;WU Jianfeng;WEN Ying;BI Hongsheng(Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China;Eye Institute of Shandong University of Traditional Chinese Medicine,Jinan 250002,Shandong Province,China;Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases,Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong,Shandong Engineering Technology Research Center of Visual Intelligence,Shandong Optometry and Adolescent Low Vision Prevention and Control Clinical Medical Research Center,Shandong Academy of Health and Myopia Prevention and Control of Children and Adolescents,Jinan 250002,Shandong Province,China;Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250002,Shandong Province,China)

机构地区：[1]山东中医药大学,山东省济南市250014 [2]山东中医药大学眼科研究所,山东省济南市250002 [3]山东省中西医结合眼病防治重点实验室,山东省高校中西医结合眼病防治技术(强化)重点实验室,山东省视觉智能工程技术研究中心,山东省眼视光与青少年视力低下防控临床医学研究中心,山东省儿童青少年健康与近视防控研究院,山东省济南市250002 [4]山东中医药大学附属眼科医院,山东省济南市250002

出　　处：《眼科新进展》2021年第11期1047-1051,共5页Recent Advances in Ophthalmology

基　　金：国家重点研发计划项目(编号2019YFC1710200,2019YFC1710204);国家自然科学基金面上项目(编号82074498)。

摘　　要：目的应用功能性近红外光谱技术(fNIRS)探讨儿童与成人视皮层区功能活动的变化。方法本研究共纳入了46位健康志愿者。将志愿者分为两组,其中儿童组28人,年龄为(9±3)岁;成人组18人,年龄为(23±4)岁。fNIRS设备采用日立ETG-4000近红外脑功能成像仪,通过端口将fNIRS设备与呈现视觉任务的控制电脑进行连接并保证在受试者接受视觉刺激时fNIRS可以同步地检测到视皮层血红蛋白含量的实时变化。视觉刺激任务为黑白棋盘格翻转刺激。将氧合血红蛋白的数据通过Matlab软件分析得出β值,每个通道均包括基线期β值和刺激期β值,利用SPSS 21.0分析软件分别对儿童组以及成人组内受试者单通道的基线期与刺激期β值进行配对样本t检验。Δβ=β值_(刺激期)-β值_(基线期)。结果除了儿童组中的通道12外,01至11通道两组受试者刺激期β值均显著高于基线期,差异均有统计学意义(均为P<0.05),儿童组受试者与视觉刺激同步的双侧初级视皮层的Δβ值均显著高于成人组(均为P<0.05);儿童组受试者与视觉刺激同步的双侧次级视皮层的Δβ值也均显著高于成人组(均为P<0.05)。结论在同样的视觉刺激下,儿童的视皮层功能活动反应程度要明显高于成人。Objective To investigate the changes of functional activities of visual cortex in children and adults using the functional near-infrared spectroscopy(fNIRS).Methods A total of 46 healthy volunteers were included in this study.These volunteers were divided into two groups children group,including 28 children aged 9±3,and adult group,including 18 adults aged 23±4.The fNIRS device used in this study is Hitachi ETG-4000 near infrared brain function imager.It was connected to the control computer presenting visual tasks through a port,to ensure that it could synchronously detect the real-time changes of hemoglobin content in the visual cortex when patients were receiving visual stimulation.The visual stimulation used the black-and-white checkerboard flip stimulation.The data of oxyhemoglobin were analyzed by MATLAB software to obtain theβvalue.Each channel included theβvalues in both the baseline and stimulation periods.SPSS 21.0 software was used to conduct paired sample t-test on theβvalue of single channel in the baseline and stimulation periods.Δβwas equal to theβvalue in the stimulation period minus theβvalue in the baseline period.Results Except for channel 12 in the children group,theβvalues of other channels in the stimulation period were all significantly higher than those in the baseline period,and the differences were statistically significant(all P<0.05),indicating that these channels had been significantly activated under visual stimulation.TheΔβvalues of bilateral primary and secondary visual cortices synchronized with visual stimulation in the children group were significantly higher than those in the adult group(all P<0.05).Conclusion Under the same visual stimulation,the functional response of children’s visual cortex is significantly better than that of adults.

关 键 词：年龄 视皮层 血流动力学 功能性近红外光谱技术 

分 类 号：R774.7[医药卫生—眼科]