FAM83D在胰腺癌中的表达及临床意义  

Expression and clinical significance of FAM83D in pancreatic cancer

作　　者：缪宇锋 张有为[2] 焦南林[3] MIAO Yufeng;ZHANG Youwei;JIAO Nanlin(Department of Medical Oncology,the First People’s Hospital of Wenling,Zhejiang Province,Wenling317500,China;Department of Medical Oncology,Xuzhou Central Hospital,Jiangsu Province,Xuzhou221009,China;Department of Pathology,Yijishan Hospital,Wannan Medical College,Anhui Province,Wuhu241001,China)

机构地区：[1]浙江省温岭市第一人民医院肿瘤内科,浙江温岭317500 [2]江苏省徐州市中心医院肿瘤内科,江苏徐州221009 [3]皖南医学院附属弋矶山医院病理科,安徽芜湖241001

出　　处：《中国医药导报》2021年第32期9-12,27,F0003,共6页China Medical Herald

基　　金：国家自然科学基金面上项目(81973346)。

摘　　要：目的探讨FAM83D在胰腺腺癌(PAAD)组织中的表达及临床意义。方法基因表达谱动态分析(GEPIA)工具在线分析FAM83D基因表达及预后,KMplot数据库进行验证;选择2018年1月至2019年12月皖南医学院附属弋矶山医院病理科PAAD术后组织蜡块60对,包括癌组织和癌旁组织,使用免疫组织化学染色(IHC)检测FAM83D蛋白的表达;FAM83D作用机制使用基因集富集分析。结果与正常组织比较,肿瘤组织中FAM83D mRNA表达明显升高(P<0.01)。与癌旁组织比较,癌组织IHC评分显著增高(P<0.01)。PAAD患者FAM83D蛋白高表达与淋巴结转移有关(P<0.05)。与FAM83D mRNA低表达组比较,FAM83D mRNA高表达组总生存期、无进展生存期明显缩短(P<0.01)。FAM83D上调与多个基因集呈正相关(P<0.05)。结论FAM83D在PAAD发生发展中具有重要作用,可望成为潜在治疗靶点和新型分子标志物。Objective To investigate the expression and clinical significance of FAM83D in pancreatic adenocarcinoma(PAAD)tissues.Methods The gene expression and prognosis of FAM83D were analyzed online by Gene Expression Profiling Interactive Analysis(GEPIA)tool,and verified by KMplot database;60 pairs of paraffin blocks were selected after PAAD operation in the Department of Pathology,Yijishan Hospital,Wannan Medical College from January 2018 to December 2019,including cancer tissues and adjacent tissues,and immunohistochemistry(IHC)was used to detect the expression of FAM83D protein;the mechanism of FAM83D was analyzed by gene set enrichment analysis.Results Compared with normal tissues,the expression of FAM83D mRNA was significantly increased in tumor tissues(P<0.01).Compared with adjacent tissues,IHC score of cancer tissues was significantly higher(P<0.01).The high expression of FAM83D protein in PAAD patients was associated with lymph node metastasis(P<0.05).Compared with the low expression of FAM83D mRNA group,the overall survival and progression-free survival in the high expression of FAM83D mRNA group were significantly shorter(P<0.01).FAM83D up-regulation was positively correlated with multiple gene sets(P<0.05).Conclusion FAM83D plays an important role in the occurrence and development of PAAD and is expected to become a potential therapeutic target and a novel molecular marker.

关 键 词：胰腺腺癌 FAM83D 预后 免疫组织化学染色 生物信息学 

分 类 号：R735.37[医药卫生—肿瘤]