miR-21-5p在口腔黏膜下纤维化癌变细胞中的表达及生物信息学分析  

Expression of miR-21-5p in oral submucosal fibrotic cancerous cells and bioinformatics analysis

作　　者：胡兆勇 谭劲[2] 陈明[2] 杨庆泽 刘艳丽 HU Zhaoyong;TAN Jin;CHEN Ming;YANG Qingze;LIU Yanli(Anatomy Experiment Center,School of Medicine,Hunan University of Chinese Medicine,Hunan Province,Changsha,410208,China;Department of Stomatology,the First Affiliated Hospital of Hunan University of Chinese Medicine,Hunan Province,Changsha,410007,China;School of Medicine,Hunan University of Chinese Medicine,Hunan Province,Changsha,410208,China)

机构地区：[1]湖南中医药大学医学院解剖实验中心,湖南长沙410208 [2]湖南中医药大学第一附属医院口腔科,湖南长沙410007 [3]湖南中医药大学医学院,湖南长沙410208

出　　处：《中国医药导报》2021年第31期34-36,41,F0004,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81874496);湖南中医药大学基础医学一流学科开放基金项目(2018JCYX07);湖南中医药大学口腔医学一级学科开放基金项目(2019KQYX02);湖南中医药大学校级科研基金项目(2019XJJJ020)。

摘　　要：目的观察miR-21-5p在口腔黏膜下纤维化癌变细胞中的表达及其生物信息学分析。方法将大鼠原代口腔黏膜上皮细胞分为实验组和正常对照组,实验组加入50μg/ml槟榔提取物诱导,构建口腔黏膜下纤维化癌变细胞模型。采用RT-qPCR技术检测两组miR-21-5p的表达,利用miRDB、TargetScan等30个数据库预测hsa-miR-21-5p的靶基因,并进行功能富集分析和信号通路富集分析。结果RT-qPCR结果显示,实验组miR-21-5p的表达高于正常对照组,差异有高度统计学意义(P<0.01)。生物信息分析结果显示,miR-21-5p靶基因预测中综合得分排前三的是Smad7、PLAG1、TGF-β1。miR-21-5p靶基因主要富集于细胞大分子代谢、细胞内细胞器等组分及蛋白质酶聚集中,信号通路则显著富集于MAPK等信号通路。结论miR-21-5p在口腔黏膜下纤维化癌变细胞中高表达。Smad7可能通过串话miR-21-5p与TGF-β介导口腔黏膜下纤维化癌变。Objective To observe the expression of miR-21-5p in oral submucosal fibrotic cancerous cells and its bioinformatics analysis.Methods The primary oral mucosal epithelial cells of rats were divided into experimental group and normal control group.The experimental group was induced with areca nut extract of 50μg/ml,and the model of oral submucosal fibrotic cancerous cells was established.RT-qPCR was used to detect the expression of miR-21-5p in the two groups,and 30 databases including miRDB and TargetScan were used to predict the target genes of hsa-miR-21-5p,and functional enrichment analysis and signal pathway enrichment analysis were performed.Results RT-qPCR results showed that the expression of miR-21-5p in the experimental group was higher than that in the control group,and the difference was highly statistically significant(P<0.01).The results of bioinformatics analysis showed that Smad7,PLAG1,and TGF-β1 ranked the top three in the comprehensive score of miR-21-5p target gene prediction.MiR-21-5p target genes were mainly enriched in cellular macromolecular metabolism,intracellular organelles,and other components and protein enzyme aggregation,while the signaling pathways were significantly enriched in MAPK and other signaling pathways.Conclusion MiR-21-5p is highly expressed in oral submucosal fibrotic cancerous cells.Smad7 may influence oral submucosal fibrotic carcinogenesis by mediating the cross-dialogue between miR-21-5p and TGF-β.

关 键 词：miR-21-5p 口腔黏膜上皮细胞 槟榔提取物 生物信息学 

分 类 号：R739.8[医药卫生—肿瘤]