FOXM1促进子宫内膜癌细胞系HEC-1B增殖、迁移和侵袭  

FOXM1 promotes proliferation,migration and invasion of endometrial cancer cell line HEC-1B

作　　者：黄博 程苾恒 解美婷 刘珊汕 魏敏 HUANG Bo;CHENG Bi-heng;XIE Mei-ting;LIU Shan-shan;WEI Min(Department of Obstetrics and Gynecology, Hubei General Hospital, Wuhan 430000, China)

机构地区：[1]湖北省人民医院妇产科,湖北武汉430000

出　　处：《基础医学与临床》2021年第12期1780-1785,共6页Basic and Clinical Medicine

摘　　要：目的探讨抑制叉头框蛋白M1(FOXM1)基因的表达对子宫内膜癌细胞增殖、迁移和侵袭的影响。方法选择人子宫内膜癌细胞系HEC-1B,将细胞分为3组:空白对照组、阴性对照组、siRNA-FOXM1组;实时荧光定量PCR检测各组细胞FOXM1、血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)mRNA表达水平;CCK8法检测各组细胞增殖能力;细胞划痕实验检测各组细胞迁移能力;Transwell小室法检测各组细胞侵袭能力;Western blot检测各组细胞VEGF和bFGF基因的蛋白表达。结果与HEECs细胞相比,HEC-1B细胞FOXM1 mRNA和蛋白表达显著升高(P<0.05);干扰FOXM1表达后,FOXM1 mRNA和蛋白表达、HEC-1B细胞增殖、迁移和侵袭能力与空白对照组和阴性对照组相比均显著降低(P<0.05);抑制FOXM1表达后,HEC-1B细胞VEGF、bFGF mRNA及蛋白表达显著降低(P<0.05)。结论抑制FOXM1的表达可以抑制HEC-1B细胞增殖、迁移及侵袭能力,其机制可能与抑制VEGF、bFGF的表达有关。Objective To explore the effect of inhibiting the expression of forkhead box protein M1(FOXM1)gene on the proliferation,migration and invasion of endometrial cancer cell line HEC-1B.Methods Human endometrial carcinoma cell line HEC-1B was selected as the subject and divided into three groups:blank control group,negative control group and siRNA-FOXM1 group.Real-time qPCR was used to detect the expression of FOXM1,vascular endothelial growth factor(VEGF)and basic fibroblast growth factor(bFGF)mRNA in cells of each group.CCK8 method was used to detect the proliferation of cells.Cell scratch test to detect the ability of cell migration in each group.Transwell chamber method was performed to detect the invasion ability of cells in each group.The protein expression of VEGF and bFGF were detected by Western blot.Results Compared with HEECs,the expression of FOXM1 mRNA and protein in HEC-1B cells was significantly increased(P<0.05).The expression of FOXM1 mRNA and protein,proliferation,migration and invasion ability of HEC-1B cells were significantly lower than those of blank control group and negative control group(P<0.05).The mRNA and protein expressions of VEGF and bFGF in hec-1b cells were significantly decreased after FOXM1 expression was inhibited(P<0.05).ConclusionsInhibiting of FOXM1 expression can inhibit the proliferation,migration and invasion of HEC-1B cells,and the mechanism might be related to inhibiting the expression of VEGF and bFGF.

关 键 词：叉头框蛋白M1 子宫内膜癌 HEC-1B细胞 血管内皮生长因子 碱性成纤维细胞生长因子 

分 类 号：R737.33[医药卫生—肿瘤]