过表达性别决定区Y框蛋白7基因对骨肉瘤细胞生长及免疫逃逸相关因子表达的影响  

Mechanism of Overexpression of Sex Determining Region Y-box 7 Gene on the Growth of Osteosarcoma Cells and the Expression of Immune Escape Related Factors

作　　者：于云祥[1] 龚泰芳[1] 刘小涛[1] 柯文[1] 李彬彬[1] 廉凯 徐进 YU Yunxiang;GONG Taifang;LIU Xiaotao;KE Wen;LI Binbin;LIAN Kai;XU Jin(The First Department of Orthopedics,Taihe Hospital,Shiyan 442000,Hubei China;Department of Orthopedics,Xiangyang Central Hospital Affiliated to Hubei University of Arts and Sciences,Xiangyang 441021,Hubei China)

机构地区：[1]湖北十堰市太和医院骨一科,湖北十堰442000 [2]湖北文理学院附属医院(襄阳市中心医院)骨科

出　　处：《中国中医骨伤科杂志》2021年第11期13-17,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：湖北省卫生计生委科研项目(JX6B36)。

摘　　要：目的:探讨过表达性别决定区Y框蛋白7(SOX7)基因对骨肉瘤MG-63细胞活力、周期、凋亡、Wnt/β-catenin信号及免疫逃逸相关VEGF和TGF-β1表达的影响。方法:将人骨肉瘤MG-63细胞分为空白组、pcDNA3.1组和pcDNA3.1-SOX7组,pcDNA3.1转染参照Lipofectamine 2000试剂盒说明书。通过CCK-8法检测pcDNA3.1-SOX7转染MG-63细胞24~72 h的细胞活力;流式细胞仪检测pcDNA3.1-SOX7转染MG-63细胞48 h的细胞周期和凋亡率。Western Blot检测SOX7、β-catenin、cyclinD1、Survivin、VEGF和TGF-β1蛋白表达。结果:pcDNA3.1-SOX7转染MG-63细胞后,细胞中SOX7蛋白表达明显高于空白组,差异有统计学意义(P<0.05)。与空白组比较,pcDNA3.1-SOX7组细胞活力明显降低,凋亡率明显升高,G_(0)/G_(1)期细胞百分比明显升高,G_(2)/M期和S期百分比明显降低,β-catenin、CyclinD1、Survivin、VEGF和TGF-β1蛋白表达明显降低,差异有统计学意义(P<0.05)。结论:过表达SOX7基因可抑制骨肉瘤MG-63细胞活力,阻碍细胞周期进程,诱导细胞凋亡,抑制免疫逃逸相关VEGF和TGF-β1表达,其机制与抑制Wnt/β-catenin信号通路有关。Objective:To investigate the effect of SOX7 overexpression on the viability,cycle,apoptosis,Wnt/β-catenin signal and the expression of immune escape-related VEGF and TGF-β1 in osteosarcoma MG-63 cells.Methods:Human osteosarcoma MG-63 cells were divided into blank group,pcDNA3.1 group and pcDNA3.1-SOX7 group.The pcDNA3.1 was transfected according to Lipofectamine 2000 kit.The viability of MG-63 cells transfected with pcDNA3.1-SOX7 for 24 to 72 h was detected by CCK-8 method,and the cell cycle and apoptosis rate of MG-63 cells transfected with pcDNA3.1-SOX7 for 48 h were detected by flow cytometry.Western Blot was used to detect the expression of SOX7,β-catenin,cyclinD1,survivin,VEGF and TGF-β1 protein.Results:The expression of SOX7 protein in MG-63 cells transfected with pcDNA3.1-SOX7 was significantly higher than that in blank group(P<0.05).Compared with the blank group,the cell viability of pcDNA3.1-SOX7 group decreased significantly,the apoptosis rate increased significantly,the percentage of G;/G;phase cells increased significantly,the percentage of G;/M phase and S phase cells decreased significantly,and the expression ofβ-catenin,cyclinD1,survivin,VEGF and TGF-β1 protein decreased significantly(P<0.05).Conclusion:Overexpression of SOX7 gene can inhibit the viability of osteosarcoma MG-63 cells,block cell cycle progression,induce apoptosis,and inhibit the expression of immune escape-related VEGF and TGF-β1.The mechanism is related to the inhibition of Wnt/β-catenin signaling pathway.

关 键 词：骨肉瘤 性别决定区Y框蛋白7基因 凋亡 免疫逃逸 

分 类 号：R738.1[医药卫生—肿瘤]