醋酸氯己定复合介孔二氧化硅改性正畸粘接树脂的体外研究  

In vitro study of chlorhexidine acetate compound mesoporous silica orthodontic modified binder resin

作　　者：邓文振 田徐腾越 李雪微 董伟 梁永强 DENG Wenzhen;TIAN XU Tengyue;LI Xuewei;DONG Wei;LIANG Yongqiang(School of Stomatology,North China University of Science and Technology,Tangshan 063000,China;State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases,West China School of Stomatology,Sichuan University,Chengdu 610041,China;Department of Stomatology,Affiliated Hospital of Hebei University,Baoding 071000,China)

机构地区：[1]华北理工大学口腔医学院,河北唐山063000 [2]口腔疾病国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医学院,四川成都610041 [3]河北大学附属医院口腔科,河北保定071000

出　　处：《口腔疾病防治》2022年第3期178-184,共7页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：河北省高等学校科学技术研究项目(QN2020438)。

摘　　要：目的探讨醋酸氯己定(chlorhexidine acetate,CHA)复合介孔二氧化硅改性正畸3M粘接树脂的抗菌性能和粘接强度。方法将不同质量分数的CHA包封于介孔二氧化硅纳米粒子(mesoporous silica nanoparti⁃cles,MSNs)中(记为CHA@MSNs),用傅里叶红外光谱仪和扫描电镜对实验所得样品进行表征分析。将3M Z350XT流动树脂分为4组,分别添加质量分数为0%、3%、5%、6.4%的CHA@MSNs,分别为A组:3M+CHA@MSNs(0%),B组:3M+CHA@MSNs(3%),C组:3M+CHA@MSNs(5%),D组:3M+CHA@MSNs(6.4%)。以万能电子材料试验机测试改性粘接剂的抗剪切强度;10倍放大镜观察牙面粘接剂残留,并计算牙面粘接剂残留指数(adhesive remnant index,ARI)。将4组改性粘接剂分别与变异链球菌共同培养,通过分光光度计测量菌液OD540值,扫描电镜观察菌斑附着量来评价粘接剂的抗菌性能。结果傅里叶红外光谱分析表明CHA已成功负载于MSNs之上;扫描电镜下可见CHA与MSNs结合后,MSNs结构发生变化,界限较为模糊且聚集为层状结构。含CHA@MSNs的B、C、D组与未含CHA@MSNs的A组相比,抗剪切强度下降,且差异具有统计学意义(P<0.05),其中D组抗剪切强度数值下降最大,而B组和C组两组间比较,差异无统计学意义(P>0.05);各组间ARI差异无统计学意义(P>0.05);菌液OD540值结果显示,A、B、C三组之间的差异有统计学意义(P<0.05),其中C组菌液OD540值最低,C组与D组之间差异无统计学意义(P>0.05)。结论添加质量分数为5%CHA@MSNs抗菌剂抗菌作用显著提高,且不影响其粘接强度。Objective The antibacterial properties and bonding strength of 3M orthodontic adhesive resin modified by chlorhexidine acetate(CHA)composite mesoporous silica were investigated.Methods CHA with different mass fractions was encapsulated in mesoporous silica nanoparticles(MSNs)(denoted CHA@MSNs).Fourier transform infrared spectroscopy(FTIR)and scanning electron microscopy(SEM)were used to characterize the samples.The 3M Z350XT flow resin was divided into 4 groups:group A:3M+CHA@MSNs(0%);group B:3M+CHA@MSNs(3%);group C:3M+CHA@MSNs(5%);and group D:3M+CHA@MSNs(6.4%),with mass scores of 0%,3%,5%,and 6.4%,respectively.The shear strength of the modified adhesive was tested by a universal electronic material testing machine,the adhesive residue was observed by a 10×magnifying glass,and the adhesive Remnant index(ARI)was calculated.The four groups of modified adhesives were cultured with Streptococcus mutans.The OD540 value of the bacterial solution was mea⁃sured by a spectrophotometer,and the amount of plaque attachment was observed by scanning electron microscopy to evaluate the antibacterial performance of the adhesives.Results Infrared spectroscopic analysis of CHA@MSNs showed that CHA was successfully loaded onto MSNs.Under scanning electron microscopy,it could be seen that,after Cha was combined with MSNs,the structure of MSNs changed,as the boundary was fuzzy and aggregated into a layered structure.A comparison of shear strength revealed a statistically significant difference between the groups containing CHA@MSNs and the groups without CHA@MSNs(P<0.05).The value of the shear strength in group D decreased the most,while there was no statistically significant difference between group B and group C(P>0.05).There was no statis⁃tical significance across all groups(P>0.05),suggesting that the addition of CHA@MSNs had little effect on the brack⁃et shedding.The OD540 value of bacterial fluid indicated that the difference among groups A,B and C was statistically significant(P<0.05),and the antibacterial effect of gro

关 键 词：正畸粘接剂 树脂改性 釉质白斑病损 抗菌材料 醋酸氯己定 介孔二氧化硅 抗菌性 剪切粘接强度 

分 类 号：R783.1[医药卫生—口腔医学]