Enhancing prime editing by Csy4-mediated processing of pegRNA  

作　　者：Yao Liu Guang Yang Shuhong Huang Xiangyang Li Xin Wang Guanglei Li Tian Chi Yulin Chen Xingxu Huang Xiaolong Wang 

机构地区：[1]Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,China [2]School of Life Science and Technology,ShanghaiTech University,Shanghai,China [3]CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Shanghai,China

出　　处：《Cell Research》2021年第10期1134-1136,共3页细胞研究（英文版）

基　　金：We thank members of Huang lab and Wang lab for helpful discussions. We thank Prof. Quanjiang Ji from ShanghaiTech University for constructive suggestions. We thank the Molecular and Cell Biology Core Facility (MCBCF) at the School of Life Science and Technology, ShanghaiTech University for providing technical support. This work is supported by the National Natural Science Foundation of China (31972526 and 31772571);as well as Local Grants (2020KW-028 and 2018KJXX-009). X.W. is a Tang Scholar at Northwest A&F University.

摘　　要：Dear Editor,The most advanced prime editor 3(PE3)system comprises the editor,a fusion protein of Cas9 H840A nickase and mutant reverse transcriptase(RTase)(hereafter termed NMRT),a prime editing guide RNA(pegRNA)and an alternative single-guide RNA(sgRNA).1 The pegRNA contains a primer binding site(PBS)and a reverse transcription(RT)template for introducing new genetic information1(Fig.1a;Supplementary information,Fig.S1a).We noted that the PBS,which is generally 10–16 nt at the 3′end of pegRNA,is complementary to part of the spacer at the 5′end of pegRNA,and their annealing is expected to cause pegRNA circularization,which can potentially hamper editing(Fig.1a;Supplementary information,Fig.S1b,c).

关 键 词：EDITOR EDITING TEMPLATE 

分 类 号：Q2[生物学—细胞生物学]