乳腺组织癌变的偏振拉曼光谱学研究  

Study on Breast Tissue Cancerization by Polarized Micro-Raman Spectroscopy

作　　者：唐金兰 包一麟 吴进锦 尚林伟 尚慧 许志兵 王慧捷 尹建华[1] TANG Jin-Lan;BAO Yi-Lin;WU Jin-Jin;SHANG Lin-Wei;SHANG Hui;XU Zhi-Bing;WANG Hui-Jie;YIN Jian-Hua(Department of Biomedical Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China)

机构地区：[1]南京航空航天大学生物医学工程系,南京210016

出　　处：《分析化学》2021年第12期2048-2054,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.61378087);江苏省六大人才高峰计划项目(No.SWYY-034);南京航空航天大学2020年研究生创新基地(实验室)开放基金项目(No.kfjj20200315)资助。

摘　　要：偏振显微拉曼光谱(Polarized micro-Raman spectra,PMRS)技术可提供样品中分子的取向信息和有序性,并且无需染色或特殊的样本制备过程。本研究将该技术用于乳腺癌的研究,分别采集正常和癌变乳腺组织的拉曼光谱,光谱分析和差异性分析结果表明,PMRS技术可使酰胺Ⅰ带以外的主要特征带差异得以显著放大,如在常规显微拉曼光谱中没有明显差异的脯氨酸和苯丙氨酸特征带(921、1032 cm^(-1))在PMRS中也有显著差异,证明PMRS技术具有更好的乳腺癌组织识别能力。研究了酰胺Ⅲ带I_(1247)/I_(1269)的光谱特性,结果表明,在癌变组织中,I_(1247)/I_(1269)在平行和垂直偏振光激发条件下都小于1;在正常组织中,I_(1247)/I_(1269)在平行和垂直偏振条件下则分别小于1和大于1,表明癌变后胶原纤维失去了原有的方向性,同时证明这与癌变后胶原纤维的羟基化即羟基化氨基酸含量增加有关。本研究结果有助于更好地阐明乳腺癌侵袭过程中诱导浸润的分子机制,同时也表明PMRS技术更具发展成为临床乳腺癌诊断的有效工具的潜力,以辅助临床诊疗侵袭性乳腺癌。The polarized micro-Raman spectra(PMRS)technique can provide information about the orientations and ordering of molecules in samples without any special sample preparation or staining.In this work,PMRS was used to collect Raman spectra of normal and cancerous breast tissues.By spectral analysis and difference analysis,it was found that PMRS could significantly amplify the differences of main characteristic bands other than amideⅠband,even the bands of proline(921 cm^(-1))and phenylalanine(1032 cm^(-1))which had unobvious difference in conventional micro-Raman spectroscopy.The results indicated that PMRS was much efficient to identify breast cancer tissue.The bimodal intensity ratio of the amideⅢband(I_(1247)/I_(1269))was lower than 1 in cancerous tissues,no matter excited with parallel or perpendicular polarized light,but it was lower and greater than 1 in normal tissue when excited with parallel and perpendicular polarized light,respectively,indicating that collagen fibers lost their original orientation in cancerous tissues.Meanwhile,it was proved that this phenomenon was related to the increase in the content of hydroxylated amino acids of collagen fibers after canceration.The information obtained in this study helped to elucidate the molecular mechanism of induced intravasation during breast cancer invasion more clearly.It suggested that PMRS had more potential to be developed as an effective tool for clinical diagnosis of breast cancer,to assist in the clinical diagnosis and treatment of invasive breast cancer.

关 键 词：偏振拉曼光谱 乳腺癌 胶原蛋白 差异性增强 

分 类 号：R737.9[医药卫生—肿瘤]