转录因子和启动子互作分析技术及其在植物应答逆境胁迫中的研究进展  

Interaction Analysis of Transcription Factors and Promoters and Its Application in Response of Plants to Stress

作　　者：王雪 王盛昊[1,2] 于冰 Wang Xue;Wang Shenghao;Yu Bing(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500;Key Laboratory of Molecular Biology,College of Heilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500 [2]黑龙江大学生命科学学院,黑龙江省普通高校分子生物学重点实验室,哈尔滨150080

出　　处：《中国农学通报》2021年第33期112-119,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目“甜菜M14品系乙二醛酶I基因抗盐能力的转录因子功能研究”(31671751);中国博士后项目“甜菜M14品系BvM14-STPK互作蛋白鉴定及其耐盐功能研究”(204968)。

摘　　要：转录因子是一类调节基因表达的重要调控蛋白,转录因子和与其结合的启动子中的相关顺式作用元件,在基因表达方面起着分子开关的作用,因此探究转录因子与启动子的相互作用尤为重要。为了研究在植物遭受逆境胁迫时,转录因子对下游靶基因的调控机制,本文综述了参与逆境胁迫的主要转录因子家族、转录因子的转录激活活性鉴定、转录因子和启动子互作分析技术及其在植物应答逆境胁迫中的应用,为全面、深入研究植物应答逆境胁迫时的基因表达调控机制提供参考。Transcription factors are important regulatory proteins that regulate gene expression. Transcription factors and the related cis-acting elements of promoters which bind to them both play the role of molecular switches in gene expression, therefore it is particularly important to explore the interaction of transcription factors with promoters. In order to study the regulation mechanism of transcription factors on downstream target genes when plants are subjected to stress, this paper summarized the main transcription factor families involved in stress, the identification of transcriptional activation activity of transcription factors, the interaction analysis technologies between transcription factors and promoters and their applications in plants’ response to stress, which could provide references for the comprehensive and in-depth study of the gene expression regulation mechanism of plants in response to stress.

关 键 词：转录因子 启动子 染色质免疫共沉淀技术 DNase I足迹法 凝胶阻滞分析 酵母单杂交技术 逆 

分 类 号：S184[农业科学—农业基础科学]