RP-HPLC法测定石榴皮提取物没食子酸、鞣花酸含量研究  

Determination of Gallic Acid,Ellagic Acid and Punicalagin in Pomegranate Peel Extract and Its Antibacterial Effect

作　　者：范高福[1] 刘寅 梁延波[1,2] 付恩桃 汤洁 FAN Gaofu;LIU Yin;LIANG Yanbo;FU Entao;TANG Jie(Hefei Technology College,Hefei Education Master Studio,Hefei 238000,China;Shanghai Haihong Industry(Group)Chaohu Jinchen Pharmaceutical Co.,Ltd.,Chaohu 238000,China)

机构地区：[1]合肥职业技术学院合肥市教育名师工作室,安徽合肥238000 [2]上海海虹实业(集团)巢湖今辰药业有限公司,安徽巢湖238000

出　　处：《湖北民族大学学报（医学版）》2021年第4期37-40,44,共5页Journal of Hubei Minzu University(Medical Edition)

基　　金：安徽省高校自然科学研究产学研重点项目(KJ2019A1117);安徽省高校学科(专业)拔尖人才学术资助重点项目(GXBJZD80);安徽省质量工程“药物制剂教学创新团队”项目(2019CXTD112);合肥市2019年学术和技术带头人及后备人选科研项目“石榴鞣花酸提取工艺与其外用凝胶剂的制备及质量标准的研究”;2021年度合肥市教育科学规划课题项目(HJG21118);合肥职业技术学院自然科学研究“学术与技术带头人”项目(2021DTR01)。

摘　　要：目的建立安徽怀远地区石榴皮中没食子酸(GA)、鞣花酸成分(EA)的含量同时检测方法。方法采用反相高效液相色谱法(RP-HPLC),Symmetry C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇(A)-0.08%磷酸溶液(B)为流动相梯度洗脱,流速1.0 mL/min,柱温30℃,波长270 nm同时检测GA和EA含量。采用SPSS 17.0对测定结果进行分析,比较不同品种石榴皮中各成分的含量差异。结果没食子酸和鞣花酸分别在6.6375~212.4000,6.6625~213.2000μg/mL呈线性关系,平均加样回收率为96.90%(RSD=0.78%,n=6)和97.90(RSD=0.63%,n=6)。结论此方法准确稳定、灵敏方便、重现性好,可作为安徽本地石榴皮部位中GA、EA成分含量的同时检测;且白玉石榴皮中GA、EA含量较红酸石榴皮的含量偏高。Objective To establish a method for simultaneous determination of gallic acid and ellagic acid punicalagin in pomegranate peel in Huaiyuan,Anhui.Method The reversed-phase high performance liquid chromatography was used,with Symmetry C18(250 mm×4.6 mm,5μm)chromatographic column,methanol(A)-0.08%phosphoric acid solution(B)as mobile phase gradient elution,flow rate 1.0 mL/min,Column temperature 30℃,detection wavelength 270 nm to detect gallic acid and ellagic acid content.SPSS 17.0 was used to analyze the measurement results and the differences in the contents of various components in different varieties of pomegranate peel were compared.Results The gallic acid and ellagic acid showed a linear relationship between 6.6375~212.4 and 6.6625~213.2μg/mL,respectively,and the average recovery rate was 96.90(RSD=0.78%,n=6)and 97.90(RSD=0.63%,n=6).Conclusion This method was accurate,stable,sensitive,convenient,and reproducible.It could be used as a simultaneous detection of the content of gallic acid and ellagic acid in the local pomegranate skin of Anhui,and the content of gallic acid and ellagic acid in the white jade pomegranate skin was higher than that of red acid.The content of pomegranate peel was high.

关 键 词：石榴皮提取物 没食子酸 鞣花酸 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]