下调RNA解螺旋酶DDX5表达对胶质瘤SHG44细胞周期和凋亡的影响及其机制  

Effect of down-regulation the expression of RNA helicase DDX5 on cell cycle and apoptosis of glioma SHG44 and its mechanism

作　　者：秦铁城 杜润爽 宋雪飞 QIN Tiecheng;DU Runshuang;SONG Xuefei(Department of Neurosurgery,Hebei Petro China Center Hospital,Langfang,Hebei 065000,China;Department of Neurosurgery,Beijing Tongren Hospital,Beijing 100005,China;Department of Neurology,Peking Union Medical College Hospital,Beijing 100730,China)

机构地区：[1]河北中石油中心医院神经外科,河北廊坊065000 [2]北京市同仁医院神经外科,北京100005 [3]北京协和医院神经内科,北京100730

出　　处：《安徽医药》2021年第12期2475-2478,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨下调RNA解螺旋酶DDX5表达对胶质瘤SHG44细胞周期和凋亡的影响及其机制。方法2018年3月至2019年4月,将体外培养的SHG44细胞分为小干扰RNA(siRNA)-阴性对照(NC)组(转染NC siRNA)和siRNA-DDX5组(转染靶向DDX5的siRNA),采用实时荧光定量聚合酶链反应(PCR)检测各组细胞中DDX5 mRNA的表达水平,流式细胞仪分别检测各组细胞周期分布和凋亡率,蛋白质印迹法检测细胞中DDX5、β-连环素、c-Myc、细胞周期蛋白D1(cyclin D1)和存活蛋白的表达水平。结果与siRNA-NC组比较,siRNA-DDX5组细胞中DDX5 mRNA[(0.25±0.02)比(0.98±0.06)]和DDX5[(0.23±0.03)比(0.68±0.05)]、β-连环素[(0.25±0.02)比(0.80±0.06)]、c-Myc[(0.37±0.03)比(0.85±0.06)]、cyclin D1[(0.30±0.02)比(0.89±0.06)]和存活蛋白[(0.42±0.03)比(0.83±0.06)]蛋白的表达水平均明显减弱,细胞在G0/G1期所占百分比明显降低[(56.15±2.28)%比(65.76±3.22)%],而在S期所占百分比[(37.26±2.03)%比(26.95±1.16)%]和细胞凋亡率[(18.95±2.23)%比(7.02±1.26)%]明显升高(P<0.05)。结论下调DDX5表达可诱导胶质瘤SHG44细胞周期阻滞于S期并促进细胞凋亡,其作用机制可能与抑制Wnt/β-连环素信号通路的活化有关。Objective To investigate the effect of down-regulated RNA helicase DDX5 expression on cell cycle and apoptosis of gli-oma SHG44 cells and its mechanism.Methods SHG44 cells were cultured and assigned into two groups:small interference RNA(siR-NA)-negative control(NC)group(transfected NC siRNA)and siRNA-DDX5 group(transfected siRNA targeting DDX5)from March 2018 to April 2019.The expression of DDX5 mRNA in each group was detected by real-time fluorescent quantitative polymerase chain reaction(PCR),the cell cycle distribution and apoptosis rate were detected by flow cytometry,and the expression levels of DDX5,β-catenin,c-Myc,cyclin D1 and Survivin proteins were measured by Western blotting.Results Compared with the siRNA-NC group,the expression levels of DDX5 mRNA[(0.25±0.02)vs.(0.98±0.06)],DDX5[(0.23±0.03)vs.(0.68±0.05)],β-catenin[(0.25±0.02)vs.(0.80±0.06)],c-Myc[(0.37±0.03)vs.(0.85±0.06)],cyclin D1[(0.30±0.02)vs.(0.89±0.06)]and Survivin[(0.42±0.03)vs.(0.83±0.06)]proteins in the siRNA-DDX5 group were significantly decreased,the percentage of cells in the G0/G1 phase was significantly reduced[(56.15±2.28)%vs.(65.76±3.22)%],while the percentage in the S phase[(37.26±2.03)%vs.(26.95±1.16)%]and the apoptotic rate[(18.95±2.23)%vs.(7.02±1.26)%]were significantly increased(P<0.05).Conclusion Down-regulation of DDX5 expression can induce glioma SHG44 cell cycle arrest in S phase and promote apoptosis,and its mechanism may be related to inhibiting the activation of Wnt/β-catenin signaling pathway.

关 键 词：神经胶质瘤 RNA解螺旋酶类 细胞凋亡 细胞增殖 侵袭 迁移 Wnt/β-连环素信号通路 

分 类 号：R739.41[医药卫生—肿瘤]