枸杞多糖与UCA1 siRNA单用或合用对MPP^(+)诱导帕金森病细胞模型的干预作用及机制研究  

Mechanism of Lycium barbarum polysaccharide and UCA1 siRNA single or combined treatment in the MPP^(+)-induced Parkinson’s disease cell model

作　　者：王立侠[1] 韩金芬[2] WANG Lixia;HAN Jinfen(Neuroelectrophysiology Room of Zhengzhou Seventh People’s Hospital,Zhengzhou 450000,China;Department of Pediatrics,the Third Affiliated Hospital of Xinxiang Medical College,Xinxiang 453003)

机构地区：[1]郑州市第七人民医院神经电生理室,郑州450000 [2]新乡医学院第三附属医院儿内科,河南新乡453003

出　　处：《中国比较医学杂志》2021年第11期55-61,共7页Chinese Journal of Comparative Medicine

基　　金：二〇一八年度河南省医学科技攻关计划项目(2018020860)。

摘　　要：目的探讨枸杞多糖与UCA1 siRNA单独或联合应用对MPP^(+)诱导的SH-SY5Y细胞增殖、凋亡的影响及机制。方法使用MPP^(+)(终浓度为0.25、0.5、1、2 mmol/L的MPP^(+)处理细胞24 h)、枸杞多糖(50、100、200、400μg/mL的枸杞多糖预处理细胞1 h后加MPP^(+)继续处理24 h)处理SH-SY5Y细胞,选择最佳MPP^(+)(1 mmol/L)及枸杞多糖作用浓度(400μg/mL)开展后续实验。实验分为对照组(细胞不经特殊处理)、MPP^(+)组(1 mmol/L的MPP^(+)处理细胞24 h)、枸杞多糖组(LBP组)(400μg/mL的枸杞多糖预处理细胞1 h后加1 mmol/L的MPP^(+)继续处理24 h)、si-UCA1组(1 mmol/L的MPP^(+)处理细胞后转染UCA1 siRNA)、枸杞多糖+si-UCA1组。MTT法检测细胞活力;流式细胞术检测细胞凋亡率、ROS及膜电位水平。结果与对照组比较,MPP^(+)组细胞存活率、膜电位显著降低,细胞凋亡率、ROS水平显著升高(P<0.05)。与MPP^(+)组比较,LBP组和si-UCA1组细胞存活率、膜电位显著升高,细胞凋亡率、ROS水平显著降低(P<0.05)。与LBP组或si-UCA1组比较,LBP+si-UCA1组细胞存活率、膜电位显著升高,细胞凋亡率、ROS水平显著降低(P<0.05)。结论枸杞多糖及抑制UCA1均可促进MPP^(+)诱导的SH-SY5Y细胞增殖,抑制凋亡,两者联合对细胞增殖、凋亡影响更显著,机制可能与抑制线粒体凋亡途径有关。Objective To investigate the effect of Lycium barbarum polysaccharide and UCA1 siRNA individually, or in combination, on the proliferation and apoptosis of SH-SY5 Y cells induced by MPP^(+)and its mechanism. Methods SH-SY5 Y cells were treated with MPP^(+)(0.25, 0.5, 1 and 2 mmol/L for 24 hours) and LBP(50, 100, 200 and 400 μg/mL Lycium barbarum polysaccharide pretreatment for 1 hour and then treated with MPP^(+)for 24 hours). The optimal concentrations of MPP^(+)(1 mmol/L) and LBP(400 μg/mL) were selected for subsequent experiments. The experiment was divided into four groups: control group(without treatment), MPP^(+)group(cells were treated with 1 mmol/L MPP^(+)for 24 hours), Lycium barbarum polysaccharide group(cells were pretreated with 400 μg/mL Lycium barbarum polysaccharide for 1 hour and then treated with 1 mmol/L MPP^(+)for 24 hours), si-UCA1 group(cells were treated with 1 mmol/L MPP^(+)and then transfected with si-UCA1) and barbarum polysaccharide+si-UCA1 group. MTT assays were used to assess cell viability and flow cytometry was used to measure the apoptosis rate, ROS level and membrane potential. Results Compared with the control group, the cell survival rate and membrane potential in the MPP^(+)group were reduced significantly and the apoptosis rate and ROS level were increased significantly(P<0.05). Compared with the MPP^(+)group, the cell survival rate and membrane potential in Lycium barbarum polysaccharide and si-UCA1 groups were increased significantly and the apoptosis rate and ROS level were decreased significantly(P<0.05). Compared with Lycium barbarum polysaccharide and si-UCA1 groups, the cell survival rate and membrane potential in the LBP+si-UCA1 group were increased significantly and the apoptosis rate and ROS level were decreased significantly(P<0.05). Conclusions Lycium barbarum polysaccharide and inhibition of UCA1 expression promote the proliferation of SH-SY5 Y cells induced by MPP^(+)and inhibit apoptosis. Their combination has a more obvious effect on cell proliferation and ap

关 键 词：帕金森 枸杞多糖 长链非编码RNA UCA1 凋亡 氧化应激 细胞模型 

分 类 号：R-33[医药卫生]