青藏高原青稞抗白粉病SSR标记分析  

Mapping of Resistance Gene to Powdery Mildew in Highland Barley on Tibetan Plateau Using SSR Markers

作　　者：杨春葆 刘仁健 YANG Chun-bao;LIU Ren-jian(State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement,Lhasa,Tibet 850002;Researh Institute of Agriculture,Tibet Academy of Agriculture and Animal Husbandry Sciences,Lhasa,Tibet 850002)

机构地区：[1]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,西藏拉萨850002 [2]西藏自治区农牧科学院农业研究所,西藏拉萨850002

出　　处：《安徽农业科学》2021年第23期137-139,共3页Journal of Anhui Agricultural Sciences

基　　金：西藏自然科学基金项目(XZ2019ZRG-87(Z))。

摘　　要：[目的]研究青藏高原青稞抗白粉病机制。[方法]以青稞基因组数据为基础www.ncbi.nlm.nih.gov/sra(accession no.SRA201388)设计200对SSR引物,标记关于抗白粉病基因位点并定位染色体。[结果]QK019和QK072两个SSR引物与抗白粉病基因HvNEWENTRY标记遗传距离较近,分别为58.2,81.4 cM,该基因位于6H染色体上。[结论]该研究可为青稞抗白粉病机制研究提供科学依据。[Objective]In order to study the resistance mechanism of Tibetan Plateau highland barley to powdery mildew.[Method]Based on the highland barley genome data submitted by this project www.ncbi.nlm.nih.gov/sra(accession no.SRA201388),200 pairs of SSR primer markers related to powdery mildew resistance gene locus were designed and locate the chromosome.[Result]The results showed that the genetic distance between QK019 and QK072 SSR primers and the powdery mildew resistance gene HvNEWENTRY marker was 58.2 and 81.4 cM,respectively,and the gene was located on chromosome 6 H.[Conclusion]This study can provide a scientific basis for the study of resistance mechanism of highland barley to powdery mildew.

关 键 词：青稞 青藏高原 白粉病 SSR 遗传距离 染色体 

分 类 号：S512.3[农业科学—作物学]