甜玉米维生素C含量的QTL定位  

QTL Mapping of Vitamin C Content in Sweet Corn

作　　者：蒋锋 闫艳 罗智佳 陈青春 张姿丽 刘鹏飞 JIANG Feng;YAN Yan;LUO Zhijia;CHEN Qingchun;ZHANG Zili;LIU Pengfei(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangzhou Key Laboratory for Research and Development of Crop Germplasm Resources,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院农业与生物学院,广东广州510225 [2]广州市特色作物种质资源研究与利用重点实验室,广东广州510225

出　　处：《广东农业科学》2021年第11期18-24,共7页Guangdong Agricultural Sciences

基　　金：广东省鲜食玉米遗传育种工程技术研究中心(粤科产学研[2016]176号);广东省重点领域研发计划(2018B020202013);广州市特色作物种质资源研究与利用重点实验室项目(202002010010)。

摘　　要：【目的】挖掘利用高维生素甜玉米种质资源,对甜玉米维生素C含量进行QTL定位。【方法】选用维生素C含量差异显著的甜玉米自交系T77(高VC)和T15(低VC)为亲本配制杂交组合T77×T15,以该组合F2作为群体,构建SSR分子标记遗传连锁图谱;结合F2各单株果穗维生素C含量,应用复合区间作图法对甜玉米维生素C含量进行QTL定位。【结果】共检测出5个相关QTL,分别位于第2、3、6染色体。在第2染色体bin 2.04~2.05区域检测到1个维生素C含量QTL(qVC-ch.2-1),加性效应值为2.23,能够解释17.16%的表型变异;在第3染色体上检测到2个维生素C含量QTL(qVC-ch.3-1、qVC-ch.3-2),分别位于bin 3.00~3.04、bin 3.06~3.07区域,加性效应值分别为1.17和1.40,表型贡献率分别为6.64%和4.15%;在第6染色体上检测到2个维生素C含量QTL(qVC-ch.6-1、qVC-ch.6-2),分别位于bin 6.05、bin 6.05~6.06区域,加性效应值分别为0.43、1.83,表型贡献率分别为6.59%和20.87%。【结论】维生素C含量的QTL定位可为选育优质高营养甜玉米品种提供一定的理论参考。【Objective】In order to exploit and utilize high vitamin sweet corn germplasm resources,QTL mapping of vitamin C content in sweet corn was carried out.【Method】The inbred lines T77(high VC)and T15(low VC),which have significant differences in vitamin C content,were used as parents to prepare the hybrid combination T77×T15,and the combination F2 was used as mapping population to construct SSR molecular marker genetic linkage map.Combined with the vitamin C content of each individual ear in F2,QTL mapping of vitamin C content in sweet corn was carried out by using compound interval mapping method.【Result】Five related QTL were detected,which were located on chromosome 2,3 and 6.One vitamin C QTL(qVC-ch.2-1)was detected in the bin2.04-2.05 region of chromosome 2,with an additive effect value of 2.23,which could explain 17.16%of phenotypic variation.Two QTL for vitamin C content(qvc-ch.3-1 and qVC-ch.3-2)were detected on chromosome 3,which were located in bin3.00-3.04 and bin3.06-3.07 respectively,with additive effect values of 1.17 and 1.40,and phenotypic contribution rates of 6.64%and 4.15%respectively.Two QTL for vitamin C content(qvc-ch.6-1 and qVC-ch.6-2)were detected on chromosome 6,which were located in 6.05 and 6.05-6.06 regions,with additive effects value of 0.43 and 1.83,and contribution rates of 6.59%and 20.87%,respectively.【Conclusion】The study on QTL mapping of vitamin C content would provide a theoretical basis for breeding sweet corn varieties with high quality and nutrition in breeding practice.

关 键 词：甜玉米 维生素C含量 QTL 分子标记选择 SSR分子标记 连锁图谱 

分 类 号：S513.032[农业科学—作物学]