云南香叶天竺葵挥发油GC-MS分析及其体外抑菌与抗炎活性研究  

Chemical Composition Analysis of Antibacterial and Anti-inflammatory Functions of Volatile Oils from Pelargonium graveolens L’Herit.

作　　者：徐雨生 郭时印 申科 刘衡 蒋智林 XU Yu-Sheng;GUO Shi-Yin;SHEN Ke;LIU Heng;JIANG Zhi-Lin(Agricultural College,Hunan Agricultural University,Changsha 410128,China;Institute of Agricultural and Garden Technology,Pu'er University,Puer 665000,Yunnan,China;School of Pharmacy and Chemistry,Dali University,Dali 671000,Yunnan,China;College of Life Sciences,Hunan University of Science and Technology,Xiangtan 411201,Hunan,China)

机构地区：[1]湖南农业大学农学院,长沙410128 [2]普洱学院农林学院,云南普洱665000 [3]大理大学药学与化学学院,云南大理671000 [4]湖南科技大学生命科学学院,湖南湘潭411201

出　　处：《中国生物化学与分子生物学报》2021年第11期1502-1509,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：云南省中青年学术与技术带头人后备人才项目(No.2014HB027);普洱学院重点科研专项规划项目(No.2020XJGH07)资助。

摘　　要：挥发油组分一般较为复杂,常具有发散解表、杀虫抗菌等作用,而对天竺葵挥发油抑菌、抗炎和抗氧化的研究报道则较少。采用水蒸气蒸馏法提取获得香叶天竺葵挥发油,气相色谱-质谱联用技术(GC-MS)和峰面积归一化法分析出其主要含有30个化合物,已鉴定的成分占总峰面积的90.26%;运用牛津杯法得出香叶天竺葵挥发油对白色念珠菌和金黄色葡萄球菌有一定抑制作用,尤其对白色念珠菌的抑制效果明显,抑菌圈直径达15.55±1.53 mm;DPPH体外清除试验判断其具有较弱的抗氧化能力;脂多糖(LPS=10.0μg/mL)诱导RAW264.7细胞炎症模型后,给予地塞米松及低、中、高剂量的香叶天竺葵挥发油,采用Elisa法检测出其能有效降低炎症细胞的IL-1β、TNF-α表达量(P<0.01),其高剂量效果与地塞米松组表达IL-1β、TNF-α含量相近。本研究成功通过GC-MS测定和鉴别香叶天竺葵挥发油化学成分,证明了香叶天竺葵挥发油有一定的抑菌活性,且有效降低炎症细胞分泌IL-1β、TNF-α,为香叶天竺葵挥发油的开发利用提供了实验依据。The components of volatile oils are generally complex,and they often have the functions of divergent dissolving surface,insecticidal and antibacterial.However,there are few reports on bacteriostasis,anti-inflammation and antioxidation roles of volatile oils of Pelargonium graveolens L’Herit..The volatile oil of Pelargonium graveolens L’Herit.was extracted by steam distillation,and GC-MS and peak area normalization analysis showed that it mainly contained 30 compounds,and the identified components accounted for 90.26%of the total peak area.The volatile oil of Pelargonium graveolens L’Herit.has a certain inhibitory effect on Candida albicans and Staphylococcus aureus,especially on Candida albicans.The diameter of the bacteriostatic zone is 15.55±1.53 mm by using the oxford cup method.Dexamethasone and low,middle and high doses of volatile oils of Pelargonium graveolens L’Herit.were given after the RAW264.7 cell inflammatory model and was induced by lipopolysaccharide(LPS=10.0μg/mL).ELISA assays showed that it could effectively reduce the expression of IL-1βand TNF-αin inflammatory cells,and the effect of high doses was similar to that of IL-1βand TNF-αin the dexamethasone group.GC-MS was successfully used to determine and identify the chemical constituents of volatile oils from Pelargonium graveolens L’Herit.in this study.We show that the volatile oil of Pelargonium graveolens L’Herit.had certain bacteriostatic activity and effectively reduces the secretion of IL-1βand TNF-ɑby inflammatory cells.It provides an experimental basis for the development and utilization of volatile oils from Pelargonium graveolens L’Herit.

关 键 词：香叶天竺葵 挥发油 气相色谱-质谱联用技术(GC-MS) 抑菌 抗炎 

分 类 号：R9[医药卫生—药学]