Demethylbelamcandaquinone B from Marantodes pumilum var.alata(Blume)Kuntze inhibits osteoclast differentiation in RAW264.7 cells  

作　　者：Haryati Ahmad Hairi Jamia Azdina Jamal Nor Ashila Aladdin Khairana Husain Noor Suhaili Mohd Sofi Norazlina Mohamed Isa Naina Mohamed Ahmad Nazrun Shuid 

机构地区：[1]Department of Biochemistry,Faculty of Medicine,Manipal University College Malaysia,Jalan Batu Hampar,Bukit Baru,75150 Melaka,Malaysia [2]Faculty of Pharmacy,Universiti Kebangsaan Malaysia,Jalan Raja Muda Abdul Aziz,50300 Kuala Lumpur,Malaysia [3]Department of Pharmacology,Faculty of Medicine,Preclinical Building,Universiti Kebangsaan Malaysia,Jalan Yaacob Latiff,Bandar Tun Razak,Cheras,56000 Kuala Lumpur,Malaysia [4]Department of Pharmacology,Faculty of Medicine,Universiti Teknologi Mara,Jalan Hospital,47000 Sungai Buloh,Selangor,Malaysia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第12期535-542,共8页亚太热带生物医学杂志（英文版）

基　　金：supported by NKEA Research Grant Scheme(NRGS)(NH1113D018-2)。

摘　　要：Objective:To investigate the bone-resorbing effect of demethylbelamcandaquinone B(Dmcq B)extracted from Marantodes pumilum var.alata on osteoclast differentiation in RAW264.7 cells.Methods:RAW264.7 macrophages were differentiated using RANKL into osteoclast-like cells.Then,they were treated with 10μg/mL Marantodes pumilum var.alata crude aqueous extract,5μg/mL dichloromethane fraction,and 0.6μg/mL Dmcq B and 0.06μg/mL estradiol.Tartrate-resistant acid phosphatase 5b(TRACP 5b)as an osteoclast phenotypic marker was determined by TRACP staining and TRACP 5b colometric assay,and bone-resorbing pits were examined.The gene expression of pro-inflammatory cytokines(TNF-αand IL-6)was measured.Moreover,the protein expressions of pro-inflammatory cytokines(TNF-αand IL-6)and estrogen receptors were evaluated.Results:Marantodes pumilum var.alata crude aqueous extract and Dmcq B inhibited RANKL-stimulated osteoclast differentiation as evidenced by size reduction of giant multinucleated osteoclast cells,decreased TRACP 5b activity as well as the subsiding of resorbed pit area compared with normal control.In addition,they reduced the gene and protein expressions of TNF-αand IL-6.Marantodes pumilum var.alata,Dmcq B,and estradiol treatments increased the protein expressions of estrogen receptors alpha and beta in osteoclasts.Conclusions:Marantodes pumilum var.alata and its active compound,Dmcq B can inhibit osteoclast differentiation.

关 键 词：Marantodes pumilum var.alata PHYTOESTROGEN RAW264.7 induced-osteoclast Bone-resorbing effect 

分 类 号：R285[医药卫生—中药学]