长链非编码RNA H19对甲状腺癌细胞凋亡的影响及其机制  

The effect of long non-coding RNA H19 on apoptosis of thyroid cancer cells and its mechanism

作　　者：李筱雨[1] 孙建平[2] 晋潇 张晓旭 李清怀[1] Li Xiaoyu;Sun Jianping;Jin Xiao;Zhang Xiaoxu;Li Qinghuai(Department of thyroid and breast surgery,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Neurosurgery,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院甲状腺乳腺外科,石家庄050000 [2]河北医科大学第二医院神经外科,石家庄050000

出　　处：《中华实验外科杂志》2021年第12期2397-2400,共4页Chinese Journal of Experimental Surgery

基　　金：2020年度河北省医学科学研究课题计划项目(20200909)。

摘　　要：目的检测长链非编码RNA H19(lncRNA H19)对甲状腺癌细胞凋亡的影响并探讨其机制。方法体外培养人甲状腺癌FTC133细胞,对其处理并分为lncRNA H19小干扰RNA(siRNA)组和阴性对照组,应用流式细胞术检测细胞凋亡水平;应用实时定量聚合酶链反应(Real-time PCR)及蛋白质印迹法(Western blot)检测凋亡相关分子B细胞淋巴瘤-2(bcl-2)、bcl-2相关X蛋白(bax)及半胱氨酰天冬氨酸特异性蛋白酶-3(Caspase-3)的mRNA及蛋白表达水平;应用Western blot检测lncRNA H19对甲状腺癌细胞凋亡的影响与磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)信号通路间的关系,两组间均数比较采用t检验。结果实验组细胞凋亡率高于对照组[(25.06±0.32)%比(7.85±0.24)%,t=96.207,P<0.01)。实验组细胞bcl-2 mRNA表达低于对照组(0.43±0.05比1.02±0.06,t=3.521,P<0.01),bax mRNA表达高于对照组(1.64±0.09比1.01±0.07,t=5.261,P<0.01),Caspase-3 mRNA表达高于对照组(1.86±0.12比1.03±0.09,t=7.342,P<0.01)。实验组细胞bax蛋白表达高于对照组(0.924±0.081比0.408±0.124,t=5.147,P<0.01),Caspase-3蛋白表达高于对照组(0.826±0.077比0.345±0.113,t=5.109,P<0.01),bcl-2蛋白质的表达明显低于对照组(0.203±0.067比0.885±0.090,t=7.368,P<0.01)。实验组p-PI3K蛋白表达水平明显低于对照组(0.189±0.021比0.798±0.081,t=7.873,P<0.01),p-Akt的蛋白表达水平明显低于对照组(0.208±0.038比0.822±0.089,t=7.423,P<0.01)。结论敲低lncRNA H19能够通过调节PI3K/Akt信号通路来促进甲状腺癌细胞发生凋亡,其在甲状腺癌的发生发展中具有重要作用。Objective To elucidate the effect of long non-coding RNA(lncRNA)H19 on the apoptosis of thyroid cancer cells and its mechanism.Methods Thyroid cancer FTC133 cells were cultured in vitro,treated and divided into lncRNA H19 small interfering RNA(siRNA)group and negative control group,and Flow cytometry was used to detect cell apoptosis;real-time quantitative polymerase chain reaction(Real-time PCR)and Western blotting were used to detect the mRNA and protein expression levels of apoptosis-related molecules B cell lymphoma/leukemia-2(bcl-2),bcl-2 associated X protein(bax)and cysteinyl aspartate-specific protease(Caspase)-3;Western blotting was used to detect the relationship between the effect of lncRNA H19 on the apoptosis of thyroid cancer cells and the phosphatidylinositol 3 kinase(PI3K)/protein kinase B(Akt)signaling pathway,mean between two groups were compared using t-test.Results The apoptosis rate in experimental group was higher than that in control group[(25.06±0.32)%compare(7.85±0.24)%,t=96.207,P<0.01).In experimental group,the bcl-2 mRNA expression was lower than that in control group(0.43±0.05 compare 1.02±0.06,t=3.521,P<0.01),the bax mRNA expression was higher than that in control group(1.64±0.09 compare 1.01±0.07,t=5.261,P<0.01)]and the Caspase-3 mRNA expression was higher than that in control group(1.86±0.12 compare 1.03±0.09,t=7.342,P<0.01).In experimental group,the expression of bax was higher than that in control group(0.924±0.081 compare 0.408±0.124,t=5.147,P<0.01)and Caspase-3 was higher than that in control group(0.826±0.077 compare 0.345±0.113,t=5.109,P<0.01),while the expression of bcl-2 protein was lower than that in control group(0.203±0.067 compare 0.885±0.090,t=7.368,P<0.01).In experimental group,the expression of p-PI3K was lower than that in control group(0.189±0.021 compare 0.798±0.081,t=7.873,P<0.01)and p-Akt was lower than that in control group(0.208±0.038 compare 0.822±0.089,t=7.423,P<0.01).Conclusion Knockdown of lncRNA H19 can promote apoptosis of thyroid cancer

关 键 词：甲状腺癌 长链非编码RNA H19 凋亡 

分 类 号：R736.1[医药卫生—肿瘤]