嘧菌酯与三种外源硒对不同生命阶段斑马鱼的联合毒性  

Joint toxicity effects of azoxystrobin and three kinds of exogenous selenium on zebrafish during different life stages

作　　者：郭志芯 毛连纲[1] 张兰[1] 张燕宁[1] 朱丽珍 蒋红云[1] GUO Zhixin;MAO Liangang;ZHANG Lan;ZHANG Yanning;ZHU Lizhen;JIANG Hongyun(Institute of Plant Protection,Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors(Plant Origin)for Agri-products Quality and Safety,Ministry of Agriculture and Rural Affairs,Beijing 100193,China)

机构地区：[1]中国农业科学院植物保护研究所,农业农村部农产品质量安全生物性危害因子(植物源)控制重点实验室,北京100193

出　　处：《农药学学报》2021年第6期1159-1167,共9页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金(31801769);国家重点研发计划(2016YFD0200500).

摘　　要：分别测定了嘧菌酯、3种外源硒(亚硒酸钠、硒代蛋氨酸和纳米硒)对水体模式生物斑马鱼不同生命阶段的单一急性毒性,以及等毒性配比的二元混合体系的联合急性毒性,并分别采用相加指数(AI)法、毒性单位(TU)法和混合毒性指数(MTI)法对其联合效应进行了评价。结果表明:斑马鱼3个生命阶段对亚硒酸钠的敏感性(以96 h-LC50(致死中浓度)mg/L表示,其中mg指硒的质量)顺序为:仔鱼(1.11 mg/L)>胚胎(1.48 mg/L)>成鱼(13.05 mg/L);对硒代蛋氨酸的敏感性:仔鱼(0.80 mg/L)>胚胎(1.03 mg/L)>成鱼(9.36 mg/L);对纳米硒的敏感性:成鱼(0.48 mg/L)>仔鱼(1.67 mg/L)>胚胎(4.32 mg/L)。除MTI法计算嘧菌酯与硒代蛋氨酸对成鱼表现为部分相加作用外,采用AI法、TU法和MTI法计算嘧菌酯与亚硒酸钠、硒代蛋氨酸和纳米硒对斑马鱼成鱼、仔鱼和胚胎的联合作用结果一致,均表现为拮抗作用。In this work,the single acute toxicity of azoxystrobin and three kinds of exogenous selenium(sodium selenite,selenomethionine and nano-selenium),and joint acute toxicity of binary mixtures in equitoxicity ratios on zebrafish(Danio rerio)during different life stages were determined,and additive index(AI)method,toxicity unit(TU)method,and mixture toxicity index(MTI)method were adopted to evaluate the joint toxicity effects,respectively.These results showed that the sensitivity of three life stages to sodium selenite(expressed as 96 h-LC50(lethal concentration)mg/L,mg is the mass of selenium)were as followed:larvae(1.11 mg/L)>embryo(1.48 mg/L)>adult zabrafish(13.05 mg/L);the sensitivity to selenomethionine were as followed:larvae(0.80 mg/L)>embryo(1.03 mg/L)>adult zabrafish(9.36 mg/L);the sensitivity to nano-selenium were as followed:adult zabrafish(0.48 mg/L)>larvae(1.67 mg/L)>embryo(4.32 mg/L).The consistent conclusion that could be drawn from three methods(AI,TU and MTI)was that the joint toxicity effects of azoxystrobin and three kinds of exogenous selenium(sodium selenite,selenomethionine and nano-selenium)on zebrafish during different life stages(embryo,larvae and adult zabrafish)were antagonistic,except for,the joint toxicity effects of azoxystrobin and selenomethionine on adult zebrafish were partially additive by MTI method.

关 键 词：嘧菌酯 亚硒酸钠 硒代蛋氨酸 纳米硒 斑马鱼 联合毒性 

分 类 号：TQ450.26[化学工程—农药化工]