基于生物信息数据库探讨山药的营养功能及作用机制  

Exploring the Nutritional Function and Mechanism of Dioscorea opposita thunb Based on Biological Database

作　　者：安莉 秦冬梅 马婧玮 郑尊涛 廖先骏 吴绪金 An Li;Qin Dongmei;Ma Jingwei;Zheng Zuntao;Liao Xianjun;Wu Xujin(Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Sciences,Zhengzhou 450002;Institute for Control of Agrochemicals,Ministry of Agriculture and Rural Affairs,Beijing 100125)

机构地区：[1]河南省农业科学院农业质量标准与检测技术研究所,郑州450002 [2]农业农村部农药检定所,北京100125

出　　处：《中国食品学报》2021年第11期174-182,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：河南省自然科学基金青年科学基金项目(202300410528)。

摘　　要：目的:利用生物信息数据库进行山药营养功能及作用机制的探讨。方法:利用中药系统药理学分析平台TCMSP获取山药化学成分和潜在作用靶点,通过Cytoscape平台构建山药-化合物-靶点网络,找出潜在的重要化合物。采用String数据库对山药所含化合物的潜在靶点进行蛋白质相互作用网络分析,筛选出山药所含化合物发挥功能作用的潜在关键靶蛋白。通过R软件对潜在作用靶标进行KEGG功能富集分析,找出发挥功能的关键代谢途径。结果:构建出山药-化合物-靶点网络,筛选出甘氨酸、丙氨酸和豆甾醇等11个与营养功能相关的重要化学成分。通过蛋白质相互作用网络分析找出氧化氢酶(CAT)和白细胞介素6(IL6)等11个关键靶蛋白。KEGG通路富集结果表明,山药所含的化合物可能是通过系统调节神经活性配体受体相互作用、碳代谢以及氨基酸的生物合成等多个关键代谢途径发挥营养功能。结论:研究结果为山药营养功能的定位提供科学依据,为系统阐明药食同源食物作用机制提供新方法。Objective:Explore the nutritional function and mechanism of Dioscorea opposita thunb.Methods:The chemical components and potential targets of Dioscorea opposita thumb were obtained from the database analysis of the Chinese medicine system pharmacology analysis platform(TCMSP).The Cytoscape platform was used to construct a Dioscorea opposita thumb-compound-target network,and topological analysis identified the potential important compounds.Using the String database to analyze the protein interaction network of the potential targets of Dioscorea opposita thumb,then screened out the potential pivotal target proteins for the function of Dioscorea opposita thumb.KEGG functional enrichment of potential targets was analyzed by R software to find out the key metabolic pathways to function.Results:The Dioscorea opposita thumb-compound-target network was constructed,and 11 important chemical components related to nutritional pharmacological functions were screened out such as glycine,alanine and stigmasterol.The protein interaction network found 11 key target proteins such as catalase(CAT)and interleukin 6(IL6).KEGG pathway enrichment results indicated that Dioscorea opposita thumb might play a nutritive pharmacological function by systematically regulating multiple key metabolic pathways of neuroactive ligand receptor interaction,carbon metabolism,and amino acid biosynthesis.Conclusion:The results provided a scientific basis for the positioning of Dioscorea opposita thumb nutritional pharmacological function,also provided a new method for the systematic elucidation of the mechanism of medicine and food homologous food.

关 键 词：药食同源 山药 功能 作用机制 

分 类 号：TP311.13[自动化与计算机技术—计算机软件与理论] TS215[自动化与计算机技术—计算机科学与技术]