CCT亚基γ在鼻咽癌组织中的表达及临床意义  

Expression of CCT subunitγin nasopharyngeal carcinoma and its clinical significance

作　　者：李刚[1] 高天生 黎骋 李冬梅[1] 梁伟[1] 黄卓[3] LI Gang;GAO Tian-sheng;LI Cheng;LI Dong-mei;LIANG Wei;HUANG Zhuo(Department of Oncology,Wuzhou Red Cross Hospital,Wuzhou 543002,Guangxi,China;Department of Pathology,Wuzhou Red Cross Hospital,Wuzhou 543002,Guangxi,China;Department of Hospital Symptoms,Wuzhou Red Cross Hospital,Wuzhou 543002,Guangxi,China)

机构地区：[1]梧州市红十字会医院肿瘤科,广西梧州543002 [2]梧州市红十字会医院病理科,广西梧州543002 [3]梧州市红十字会医院院感科,广西梧州543002

出　　处：《川北医学院学报》2021年第11期1415-1419,共5页Journal of North Sichuan Medical College

基　　金：广西壮族自治区卫生厅自筹科研课题(z2012702)。

摘　　要：目的:探讨CCT亚基γ在鼻咽癌组织中的表达及临床意义。方法:收集100例鼻咽癌组织(鼻咽癌组)和60例鼻咽黏膜慢性炎组织(鼻咽黏膜慢性炎组),实时荧光定量PCR(RT-qPCR)检测CCT亚基γmRNA表达水平,免疫组化及免疫印迹检测组织标本中CCT亚基γ蛋白表达水平,单因素分析CCT亚基γ与鼻咽癌患者临床病理特征及预后的关系。结果:鼻咽癌组CCT亚基γmRNA表达水平、蛋白阳性表达率评分及蛋白水平均高于鼻咽黏膜慢性炎组(P<0.05)。CCT亚基γ蛋白表达水平与鼻咽癌患者的TNM分期、复发有关(P<0.05),与年龄、性别、吸烟史、远处转移及病理分型无关(P>0.05)。CCT亚基γ高表达水平患者的3年总生存率低于低表达水平患者(P<0.05)。结论:CCT亚基γ在鼻咽癌组织高表达与鼻咽癌患者临床分期及复发密切相关,CCT亚基γ增高预示着患者预后不良。Objective:To explore the expression of CCT subunitγin nasopharyngeal carcinoma and its clinical significance.Methods:A total of 100 cases of nasopharyngeal carcinoma(nasopharyngeal carcinoma group)and 60 cases of nasopharyngeal mucosal chronic inflammation(nasopharyngeal mucosal chronic inflammation group)were collected,the expression level of CCT subunitγmRNA was detected by quantitative real-time PCR(RT-qPCR),and the expression level of CCT subunitγprotein in tissue samples was detected by immunohistochemistry and Western blotting.The relationship between CCT subunitγand clinicopathological characteristics of NPC patients and the prognosis of NPC patients was analyzed by univariate analysis.Results:The mRNA expression,the positive expression rate score and protein level of CCT subunitγin nasopharyngeal carcinoma group were significantly higher than those in nasopharyngeal mucosal chronic inflammation group(P<0.05).The expression level of CCT subunitγwas significantly correlated with TNM stage and recurrence of NPC patients(P<0.05),but not with age,sex,smoking history,distant metastasis and pathological classification(P>0.05).The 3-year overall survival rate of patients with high expression of CCT subunitγwas lower than that of patients with low expression of CCT subunitγ(P<0.05).Conclusion:The high expression of CCT subunitγin nasopharyngeal carcinoma tissues is closely related to clinical stage and recurrence of nasopharyngeal carcinoma patients,and the increase of CCT subunitγis predictive of poor prognosis.

关 键 词：鼻咽癌 鼻咽黏膜慢性炎 CCT亚基γ MRNA 蛋白 临床病理特征 生存分析 

分 类 号：R739.63[医药卫生—肿瘤]