饲粮大麦粉碎粒度对湖羊瘤胃微生物组成及肌肉脂肪酸的影响  

Effect of dietary barley particle size on rumen microflora and muscle fatty acids in Hu sheep

作　　者：马晓文 李发弟[1,2] 李飞 郭龙[1] MA Xiao-wen;LI Fa-di;LI Fei;GUO Long(State Key Laboratory of Grassland Agro-ecosystem,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China;Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province,Minqin 733300,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃兰州730020 [2]甘肃省肉羊繁育生物技术工程实验室,甘肃民勤733300

出　　处：《草业学报》2021年第12期202-211,共10页Acta Prataculturae Sinica

基　　金：农牧交错带牛羊牧繁农育关键技术集成示范项目(16200158);长江学者和创新团队发展计划(IRT_17R50);甘肃现代农业(草食畜)产业技术体系(GARS-09)资助。

摘　　要：将大麦进行不同粒度粉碎处理改变瘤胃可降解淀粉的含量,研究其对湖羊血常规、瘤胃微生物组成及肌肉脂肪酸的影响。试验选取54只健康3月龄湖羊公羔,随机分为3个处理,分别为大麦经2、3和4 mm的筛片粉碎,每个处理18个重复,每个重复1只羊。整个试验期包括预饲期7 d,正试期63 d和采样期1 d。试验结果表明:1)大麦粉碎处理对湖羊血液的红细胞总数、血红蛋白浓度、红细胞压积、血小板总数、淋巴细胞计数、单核细胞计数、嗜酸性粒细胞计数、嗜中性粒细胞计数和嗜碱性粒细胞计数等血常规指标均无显著影响(P>0.05)。2)大麦4 mm粉碎组总菌和溶纤维丁酸弧菌数量显著高于3 mm粉碎组(P<0.05),反刍兽新月形单胞菌数量显著低于2和3 mm粉碎组(P<0.05),产琥珀酸丝状杆菌数量显著高于2 mm粉碎组(P<0.05)。普雷沃氏菌、白色瘤胃球菌和黄色瘤胃球菌等微生物的数量各组间差异不显著(P>0.05)。3)大麦4 mm粉碎组湖羊肌肉中的C10:0、C12:0、C14:0和C14:1含量均显著高于2 mm粉碎组(P<0.05),C18:2n-9t11t和共轭亚油酸(conjugated linoleic acid,CLA)含量显著高于3 mm粉碎组(P<0.05),肌肉中anteiso C15:0、C16:1、C18:2n6t和C18:2n-9c11t的含量随着粉碎粒度的增加有增加的趋势(0.05≤P<0.10)。综上,当饲喂湖羊4 mm粉碎大麦的饲粮时,湖羊瘤胃内容物中纤维分解菌数量增加,反刍兽新月形单胞菌数量减少,肌肉脂肪酸中CLA含量增加,因此,4 mm粉碎处理对湖羊瘤胃微生物区系和肌肉脂肪酸CLA合成的影响效果最佳。The purpose of this experiment was to study the changes in the content of rumen degradable starch when crushed barley with different particle sizes was included in experiment diets of Hu sheep,and to study the effects on routine blood test results,rumen microbial composition and muscle fatty acids.In the experiment,54 healthy 3-month-old Hu lambs were randomly divided into 3 treatment groups,with 18 animals(replicates)in each group and one sheep per replicate.The treatments comprised barley ground with 2,3 or 4 mm sieve apertures.The experiment included a seven-day pre-feeding period,a 63-day fattening period and a one-day sampling period.The experiment results showed that:1)The different ground particle size of barley had no effect on the total numbers of red blood cells,hemoglobin concentration,hematocrit,platelets,lymphocytes,monocytes,eosinophils,neutrophils or basophils(P>0.05).2)For sheep fed barley ground to 4 mm,the total number of rumen bacteria and Butyrivibrio fibrisolvens was significantly higher than for sheep fed barley ground to 3 mm(P<0.05),the population of Succinimonas amylolytica was significantly lower than for sheep fed barley ground to 2 and 3 mm(P<0.05),and the population of Fibrobacter succinogenes was significantly higher than for the sheep fed barley ground to 2 mm(P<0.05).There was no significant difference among the groups in the numbers of rumen microorganisms such as Prevotella brevis,Ruminococcus albus and Ruminococcus flavefaciens(P>0.05).3)For sheep fed barley ground to 4 mm,the muscle contents of C10:0,C12:0,C14:0 and C14:1 fatty acids were significantly higher than those of sheep fed barley ground to 2 mm(P<0.05),and the contents of C18:2n-9t11t acids and conjugated linoleic acid(CLA)were significantly higher than sheep fed barley ground to 3 mm(P<0.05).Meanwhile,anteiso C15:0,C16:1,C18:2n6t and C18:2n-9c11t acid concentrations of muscles tended to increase with increase in barley feed particle size increasing(0.05≤P<0.10).In summary,when Hu sheep were fed a diet of crushed ba

关 键 词：大麦 粉碎 湖羊 瘤胃微生物 肌肉脂肪酸 

分 类 号：S826.5[农业科学—畜牧学]