CircKIF4A靶向调控miR-384表达对卵巢癌SKOV3细胞增殖和凋亡的影响  

The effect of circKIF4A on the proliferation and apoptosis of ovarian cancer SKOV3 cells through targeted regulation of miR-384 expression

作　　者：蒙秋[1] 黄守国[1] MENG Qiu;HUANG Shouguo(Department of Obstetrics and Gynecology,Haikou Hospital Affiliated to Xiangya Medical College of Central South University,Hainan 570208,China)

机构地区：[1]中南大学湘雅医学院附属海口医院妇产科,570208

出　　处：《中国妇产科临床杂志》2021年第6期635-638,共4页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：2021年海南省基础与应用研究计划(省自然科学基金)项目(820QN424)。

摘　　要：目的探讨circKIF4A对卵巢癌SKOV3细胞增殖、凋亡的影响及其对miR-384的调控作用机制。方法采用q RT-PCR法检测卵巢癌组织与癌旁组织中circKIF4A、miR-384的表达量;分别将si-NC、sicircKIF4A、miR-NC、miR-384 mimics、si-circKIF4A与anti-miR-NC、si-circKIF4A与anti-miR-384转染入SKOV3细胞;采用q RT-PCR法检测SKOV3细胞中circKIF4A、miR-384的表达量;采用MTT实验与流式细胞术分别检测细胞增殖及凋亡能力;双荧光素酶报告实验检测circKIF4A与miR-384的靶向关系。结果与癌旁组织比较,卵巢癌组织中circKIF4A的表达水平升高[(1.00±0.06),(4.28±0.32)](t=62.915, P <0.05),miR-384的表达水平降低[(1.00±0.05),(0.43±0.03)](t=61.047, P <0.05);转染si-circKIF4A后SKOV3细胞OD值降低[(0.75±0.05),(0.41±0.03)](t=17.493, P <0.05),凋亡率升高[(6.36±0.53)%,(23.19±2.21)%](t=22.216,P <0.05);转染miR-384 mimics后SKOV3细胞OD值降低[(0.73±0.05),(0.47±0.04)](t=12.182, P <0.05),凋亡率升高[(7.53±0.41)%,(19.11±1.06)%](t=30.567, P <0.05);双荧光素酶报告实验证实circKIF4A能够吸附miR-384,并可充当miR-384的海绵分子;共转染si-circKIF4A与anti-miR-384后SKOV3细胞OD值升高[(0.40±0.04),(0.65±0.03)](t=15.000, P <0.05),凋亡率降低[(25.20±2.21)%,(10.37±0.86)%](t=18.761, P <0.05)。结论抑制circKIF4A表达可负向调控miR-384的表达从而抑制卵巢癌细胞增殖及诱导细胞凋亡。Objective To explore the effect of circKIF4A on the proliferation and apoptosis of ovarian cancer SKOV3 cells and its regulatory mechanism on miR-384. Methods The qRT-PCR method was used to detect the expression of circKIF4A and miR-384 in ovarian cancer tissues and adjacent tissues. si-NC, si-circKIF4A, miR-NC, miR-384 mimics, sicirc KIF4 A and anti-miR-NC, si-circKIF4A and anti-miR-384 were transfected into SKOV3 cells respectively. The qRT-PCR method was used to detect the expression of circKIF4A and miR-384 in SKOV3 cells. MTT experiment and flow cytometry experiment were used to detect cell proliferation and apoptosis respectively. The dual luciferase reporter experiment was used to detect the targeting relationship between circKIF4A and miR-384. Results Compared with adjacent tissues, the expression level of circKIF4A in ovarian cancer tissues was increased [(1.00 ± 0.06),(4.28 ± 0.32)](t = 62.915, P < 0.05), and the expression level of miR-384 was decreased [(1.00 ± 0.05),(0.43 ± 0.03)](t = 61.047, P < 0.05). After transfection with sicirc KIF4 A, the OD value of SKOV3 cells was decreased [(0.75 ± 0.05),(0.41 ± 0.03)](t = 17.493, P < 0.05), and the apoptosis rate was increased [(6.36 ± 0.53)%,(23.19 ± 2.21)%](t = 22.216, P < 0.05). After transfection of miR-384 mimics, the OD value of SKOV3 cells was decreased [(0.73 ± 0.05),(0.47 ± 0.04)](t = 12.182, P < 0.05), and the apoptosis rate was increased [(7.53 ± 0.41)%,(19.11) ± 1.06)%](t = 30.567, P < 0.05). The dual luciferase report experiment confirmed that circKIF4A could adsorb miR-384 and can act as a sponge molecule for miR-384. After co-transfection with si-circKIF4A and anti-miR-384, the OD value of SKOV3 cells was increased [(0.40 ± 0.04),(0.65 ± 0.03)](t = 15.000, P < 0.05), and the apoptosis rate was decreased [(25.20 ± 2.21)%,(10.37 ± 0.86)%](t = 18.761, P < 0.05). Conclusion Inhibition of circKIF4A expression could negatively regulate the expression of miR-384, thereby inhibiting the proliferation of ovarian cancer cells and induc

关 键 词：circKIF4A miR-384 卵巢癌 增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]