谷胱甘肽硫转移酶M1和谷胱甘肽硫转移酶M2在甲状腺滤泡癌中的表达及其意义  

Expressions of glutathione S-transferases M1 and glutathione S-transferases M2 in follicular thyroid carcinoma and their significances

作　　者：薄剑波 吕春晖 高峰[2] 蔺广荣 Bo Jianbo;Lyu Chunhui;Gao Feng;Lin Guangrong(Department of General Surgery 1,Dandong First Hospital of Liaoning Province,Dandong 118000,China;Department of Thyroid Surgery,Binzhou People's Hospital of Shandong Province,Binzhou 256610,China)

机构地区：[1]辽宁省丹东市第一医院普外一科,118000 [2]山东省滨州市人民医院甲状腺外科,256610

出　　处：《肿瘤研究与临床》2021年第10期737-741,共5页Cancer Research and Clinic

基　　金：山东省滨州市人民医院科研启动基金(YBKTZR202114)。

摘　　要：目的探讨谷胱甘肽硫转移酶M1(GSTM1)和谷胱甘肽硫转移酶M2(GSTM2)在甲状腺滤泡癌(FTC)中的表达及其临床意义。方法收集基因表达综合(GEO)数据库中甲状腺滤泡性肿瘤基因芯片GSE82208数据,共52例样本,包括FTC 27例,甲状腺滤泡性腺瘤(FA)25例。提取基因矩阵数据并整理,利用R语言Limma包筛选出FTC和FA间差异表达基因。收集2000年1月至2020年12月辽宁省丹东市第一医院手术切除的FTC及FA标本各56例。采用免疫组织化学SABC法检测FTC和FA标本中GSTM1、GSTM2蛋白的表达水平,分析二者与FTC患者临床病理因素间的关系及二者间的相互关系。结果基于GEO数据库数据,共获得40个FTC和FA间差异表达基因;其中FTC中表达上调9个,分别为GSTM1、GSTM2、COL6A2、CUX2、CLUH、TSC2、OGDHL、ACADVL、SDHA;表达下调31个。免疫组织化学法检测显示,在手术切除FTC标本中,GSTM1、GSTM2阳性率分别为71.4%(40/56)、80.4%(45/56),在FA中阳性率分别为23.2%(13/56)、14.3%(8/56),差异均有统计学意义(χ2值分别为26.11、49.03,均P<0.01)。FTC中GSTM1和GSTM2蛋白表达与临床分期、浸润程度及远处转移均有关(均P<0.05),与性别、年龄和肿瘤长径均无关(均P>0.05)。FTC中GSTM1和GSTM2表达呈正相关(r=0.384,P=0.004)。结论FTC中GSTM1和GSTM2表达水平升高,二者可能相互作用,参与FTC的发生、发展。Objective To investigate the expressions of glutathione S-transferases M1(GSTM1)and glutathione S-transferases M2(GSTM2)in follicular thyroid carcinoma(FTC)and their clinical significances.Methods Gene expression profile of GSE82208 generated from 52 human thyroid samples,including 27 cases of FTC and 25 cases of follicular adenoma(FA)were collected from Gene Expression Omnibus(GEO)database.The gene matrix data were extracted and analyzed,and then differentially expressed genes(DEG)between FTC and FA were identified by using Limma package.Immunohistochemical SABC method was used to detect the expression levels of GSTM1 and GSTM2 proteins in FTC tissues and FA tissues collected from 56 FTC samples and 56 FA samples in Dandong First Hospital of Liaoning Province from January 2000 to December 2020.The relationship between GSTM1 and GSTM2 was analyzed;the association of expression levels of GSTM1 and GSTM2 with the clinicopathological factors of FTC patients was also analyzed.Results Based on the GEO database,a total of 40 DEG were identified,including 9 up-regulated DEG(GSTM1,GSTM2,COL6A2,CUX2,CLUH,TSC2,OGDHL,ACADVL,SDHA)and 31 down-regulated DEG in FTC.The immunohistochemistry results of samples resected showed that the positive rates of GSTM1 and GSTM2 proteins in FTC tissues were higher than those in FA tissues[71.4%(40/56)vs.23.2%(13/56),80.4%(45/56)vs.14.3%(8/56)],and differences were statistically significant(χ2 values were 26.11 and 49.03,both P<0.01).The expressions of GSTM1 and GSTM2 in FTC tissues were correlated with clinical staging,invasion degree and distant metastasis(all P<0.05),but not with gender,age and tumor diameter(all P>0.05).There was a positive correlation between GSTM1 and GSTM2 proteins expressions in FTC(r=0.384,P=0.004).Conclusions The expression levels of GSTM1 and GSTM2 in FTC are increased.The interaction between GSTM1 and GSTM2 proteins can be involved in the development and progression of FTC.

关 键 词：甲状腺肿瘤 腺癌 滤泡性 抗药性 肿瘤 计算生物学 谷胱甘肽转移酶 谷胱甘肽硫转移酶M1 谷胱甘肽硫转移酶M2 

分 类 号：R736.1[医药卫生—肿瘤]