大鼠正畸牙移动过程中坏死性凋亡对牙周组织中IL-1β及IL-17的影响  

Effect of necroptosis on IL-1β and IL-17 in periodontal membrane during orthodontic tooth movement in rats

作　　者：陈思言 季开心 张敏杰 唐林[1] 张苗苗[1] CHEN Siyan;JI Kaixin;ZHANG Minjie;TANG Lin;ZHANG Miaomiao(Department of Orthodontics,School of Stomatology,Harbin Medical University,Harbin 150000f China)

机构地区：[1]哈尔滨医科大学口腔医学院正畸科,黑龙江哈尔滨150000

出　　处：《口腔医学》2021年第11期977-982,共6页Stomatology

基　　金：哈医大一院科研创新基金(2017Y022)。

摘　　要：目的通过构建大鼠正畸牙移动模型探究大鼠牙移动过程中坏死性凋亡对牙周膜中IL-1β、IL-17表达的影响。方法80只雄性SD大鼠随机分为四组:空白对照组、抑制剂组、加力组、加力+抑制剂组,各组分别根据建模时间再随机分为1、3、5、7、14 d五个亚组,建立大鼠左侧上颌第一磨牙正畸模型,游标卡尺测量大鼠上颌第一磨牙向近中移动的距离,取压力侧牙周组织,HE染色观察压力侧牙周组织病理变化,免疫组织化学染色方法检测IL-1β、IL-17的表达。结果 IL-1β、IL-17的表达均随加力时间推移呈先增高后降低趋势(P<0.05)。与加力组相比较,加力+抑制剂组牙周组织中IL-1β、IL-17表达下调(P<0.05)。结论抑制坏死性凋亡会下调炎性因子分泌水平,正畸牙齿移动过程中坏死性凋亡可能参与压力侧牙周组织细胞死亡,影响破骨细胞分化,影响正畸牙齿移动。Objective To study the effect of necroptosis on the expression of IL-1β and IL-17 in periodontal membrane during orthodontic tooth movement in rats.Methods Eighty male SD rats were randomly divided into four groups:control group, inhibitor group,force group and force+inhibitor group. Rats in each group were randomly divided into 1, 3, 5, 7 and 14 d five subgroups according to the modeling time. Rats were used to establish models for orthodontic force applied on the left side of the maxillary first molars. The moving distance of the maxillary first molar was measured with a vernier caliper. HE staining was used to observe the pathological changes of periodontal tissue on the pressure side. The periodontal tissues on the pressure side of the rats were collected and the expression of IL-1β and IL-17 was detected by immunohistochemical staining.Results The expressions of IL-1β and IL-17 in the pressure side of periodontal tissue were firstly increased and then decreased as the time of stress treatment increased(P<0.05). Compared with the force group at the same time point, the positive expression of IL-1β and IL-17 in the periodontal tissues of the force+inhibitor group was down-regulated(P<0.05).Conclusion Inhibition of necroptosis can down-regulate the secretion of inflammatory factors. Necroptosis may be involved in the death of periodontal cells in the pressure side of tooth movement and affect the differentiation of osteoclasts and orthodontic tooth movement.

关 键 词：正畸牙移动 坏死性凋亡 白介素1Β 白介素17 

分 类 号：R783.5[医药卫生—口腔医学]