沉默PROX1基因对甲状腺乳头状癌TPC-1细胞的影响  

Effect of silencing PROX1 gene on TPC-1 cells of papillary thyroid carcinoma

作　　者：刘菲 张晨曦 张苗苗 孙小莉[1] 韩晨博[2] 王佳 LIU Fei;ZHANG Chen-xi;ZHANG Miao-miao;SUN Xiao-li;HAN Chen-bo;WANG Jia(Department of Pathology,Baoji Maternal and Child Health Care Hospital,Baoji Shaanxi 721000,China;Department of Comprehensive Surgery,Baoji Maternal and Child Health Care Hospital,Baoji Shaanxi 721000,China;Department of Pathology,Xi’an People’s Hospital,Xi’an Shaanxi 710000,China)

机构地区：[1]宝鸡市妇幼保健院病理科,陕西宝鸡721000 [2]宝鸡市妇幼保健院综合外科,陕西宝鸡721000 [3]西安市人民医院病理科,陕西西安710000

出　　处：《局解手术学杂志》2021年第12期1021-1024,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：陕西省科学技术研究发展计划项目(2018JM014)。

摘　　要：目的探讨沉默prospero相关同源异形盒蛋白1(PROX1)基因对甲状腺乳头状癌(PTC)TPC-1细胞增殖、侵袭、迁移和凋亡的影响。方法将TPC-1细胞分别转染NC-siRNA(NC-siRNA组)和PROX1-siRNA(PROX1-siRNA组)。Western blot检测PTC细胞系BCPAP、TPC-1、K1、IHH4和正常甲状腺滤泡上皮细胞系Nthy-ori-3-1中PROX1蛋白表达水平。CCK-8法检测细胞增殖能力;Transwell实验检测细胞侵袭能力;细胞划痕实验检测细胞迁移能力;Annexin V-FITC/PI流式细胞术检测细胞凋亡情况。Western blot检测Notch1信号通路相关蛋白(hes1、hes5、NICD)表达水平。结果与正常甲状腺滤泡上皮细胞系Nthy-ori-3-1比较,PTC细胞系TPC-1、BCPAP、K1和IHH4中PROX1蛋白表达水平明显较高(P<0.05),其中TPC-1最高。与NC-siRNA组比较,PROX1-siRNA组PROX1蛋白表达水平及细胞增殖、侵袭、迁移能力较低,细胞凋亡率较高(P<0.05)。与NC-siRNA组比较,PROX1-siRNA组hes1、hes5和NICD蛋白表达水平较低(P<0.05)。结论沉默PROX1可通过抑制Notch1信号通路进而抑制TPC-1细胞的增殖、侵袭、迁移,并促进其凋亡。Objective To investigate the effect of silencing prospero-related homeobox 1(PROX1)gene on the proliferation,invasion,migration and apoptosis of papillary thyroid carcinoma(PTC)TPC-1 cells.Methods The TPC-1 cells were transfected with NC-siRNA(the NC-siRNA group)and PROX1-siRNA(the PROX1-siRNA group),respectively.Western blot was used to detect the expression levels of PROX1 protein in the PTC cell lines BCPAP,TPC-1,K1,IHH4 and normal thyroid follicular epithelial cell line Nthy-ori-3-1.CCK-8 method was used to detect the cell proliferation ability;Transwell test was used to detect the cell invasion ability;cell scratch test was used to detect the cell migration ability;Annexin V-FITC/PI flow cytometry was used to detect the cell apoptosis ability.Western blot was used to detect the expression levels of Notch1 signaling pathway-related proteins(hes1,hes5 and NICD).Results Compared with the normal thyroid follicular epithelial cell line Nthy-ori-3-1,the expression levels of PROX1 protein in the PTC cell lines TPC-1,BCPAP,K1 and IHH4 were significantly higher(P<0.05),with TPC-1 being the highest.Compared with the NC-siRNA group,the PROX1-siRNA group had lower PROX1 protein expression level,cell proliferation ability,invasion ability and migration ability,and higher cell apoptosis rate(P<0.05).Compared with the NC-siRNA group,the protein expression levels of hes1,hes5 and NICD in the PROX1-siRNA group were lower(P<0.05).Conclusion Silencing PROX1 can inhibit the proliferation,invasion and migration of TPC-1 cells and promote their apoptosis by inhibiting the Notch1 signaling pathway.

关 键 词：prospero相关同源异形盒蛋白1 甲状腺乳头状癌 增殖 侵袭 迁移 凋亡 

分 类 号：R736.1[医药卫生—肿瘤]