七氟烷抑制宣威肺癌XWLC-05细胞生物学行为  

Sevoflurane Inhibits Xuanwei Lung Cancer Cell Line XWLC-05

作　　者：全宇航 王忠慧[1] 李珊珊[1] 刘光顺[1] QUAN Yu-hang;WANG Zhong-hui;LI Shan-shan;LIU Guang-shun(Dept.of Anesthesiology,The 3rd Affiliated Hospital of Kunming Medical University/Cancer Hospital of Yunnan Province,Kunming Yunnan 650118,China)

机构地区：[1]昆明医科大学第三附属医院/云南省肿瘤医院麻醉科,云南昆明650118

出　　处：《昆明医科大学学报》2021年第12期1-5,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2018FE001-070;2019FE001-248)。

摘　　要：目的了解七氟烷对宣威肺癌细胞XWLC-05增殖、迁移、侵袭及恶性行为的影响,并进一步探讨其潜在机制。方法七氟烷对XWLC-05细胞进行处理,体外实验检测XWLC-05细胞增殖、凋亡、迁移和侵袭变化;体内实验检测XWLC-05细胞致瘤性,蛋白印迹法分析增殖关键分子PI3K/AKT、细胞侵袭蛋白MMP-2/MMP-9的表达。结果对照组的克隆形成数(287.5±23.1)个,高于实验组(174.1±16.3)个,(P<0.05);对照组的凋亡率(1.60±0.38)%,低于实验组(31.48±5.81)%,(P<0.01);对照组迁移细胞数(87.8±10.2)%,高于实验组(35.2±4.4)%,(P<0.05);对照组侵袭细胞数(93.6±7.3)个,高于实验组(30.7±3.2)个,(P<0.01)。七氟烷处理下调增殖蛋白分子PI3K、p-AKT和侵袭蛋白分子MMP-2、MMP-9的表达水平(P<0.05)。结论七氟烷通过抑制PI3K/AKT信号通路降低宣威肺癌细胞XWLC-05的增殖能力,诱导细胞凋亡,减弱XWLC-05细胞的迁移、侵袭和成瘤能力。七氟烷可能成为宣威肺癌治疗的一种新方法。Objective To investigate the effects of sevoflurane on proliferation,migration,invasion and malignant behavior of Xuanwei lung cancer cells XWLC-05,and to further explore its potential mechanism.Method XWLC-05 cells were treated with sevoflurane,and the changes of proliferation,apoptosis,migration and invasion of XWLC-05 cells were detected in vitro;the tumorigenicity of XWLC-05 cells was detected in vivo,and the expressions of PI3K/AKT and MMP-2/MMP-9 were analyzed by Western blot.Result The number of clones in control group(287.5±23.1)was higher than that in experimental group(174.1±16.3)(P<0.05);the apoptosis rate in the control group(1.60±0.38)was significantly lower than that in the experimental group(31.48±5.81)(P<0.01),and the number of migrating cells in the control group(87.8±10.2)was significantly higher than that in the experimental group(35.2±4.4)(P<0.05).The number of invasive cells in the control group(93.6±7.3)was significantly higher than that in the experimental group(30.7±3.2)(P<0.01).Sevoflurane treatment significantly decreased the expression levels of PI3K,p-Akt and MMP-2,MMP-9(P<0.05).Conclusion By inhibiting PI3K/AKT signaling pathway,sevoflurane decreased the proliferation ability of XWLC-05,induced apoptosis,and weakened the migration,invasion and tumorigenesis ability of XWLC-05 cells.Sevoflurane may be a new treatment option for Xuanwei lung cancer.

关 键 词：七氟烷 宣威肺癌 细胞凋亡 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤] R730.2[医药卫生—临床医学]