血浆环状RNA hsa_circ_0004812在HBV相关性肝癌中的诊断价值  

Plasma circular RNA hsa_circ_0004812 is a diagnostic biomarker for hepatitis B virus-related hepatocellular carcinoma

作　　者：郭靓 赵鑫[1] 强雪芹 GUO Liang;ZHAO Xin;QIANG Xueqin(Department of Laboratory,Chengdu Seventh People's Hospital,Chengdu,Sichuan 610213,China)

机构地区：[1]成都市第七人民医院检验科,四川省成都市610213

出　　处：《中南医学科学杂志》2021年第6期659-663,共5页Medical Science Journal of Central South China

基　　金：四川省卫生和计划生育委员会科研课题(17PJ413)。

摘　　要：目的探讨血浆环状RNA(circRNA)hsa_circ_0004812在乙型肝炎病毒(HBV)相关性肝癌中的诊断价值。方法选择HBV相关性肝癌(肝癌组)、慢性乙型肝炎(肝炎组)、乙型肝炎肝硬化(肝硬化组)患者,设同期健康志愿者为正常对照组。采用qRT-PCR检测血浆hsa_circ_0004812表达,并建立受试者操作特征(ROC)曲线,评估hsa_circ_0004812与AFP联合检测对肝癌的诊断价值。结果HBV相关性肝癌患者中hsa_circ_0004812表达明显高于正常对照组、肝炎组和肝硬化组(P<0.05)。ROC曲线显示hsa_circ_0004812和甲胎蛋白均具有较高的诊断效能(AUC>0.7),且hsa_circ_0004812和甲胎蛋白联合诊断效能更高(AUC 0.834~0.853)。结论circRNA(hsa_circ_0004812)是一个潜在的HBV相关性肝癌诊断生物标志物,且与AFP联合诊断有更高的鉴别能力。Aim To investigate the expression of plasma circular RNA(circRNA)hsa_circ_0004812 in the diagnosis of hepatitis B virus(HBV)-related liver cancer.Methods Patients with HBV related liver cancer(HCC group),chronic hepatitis B(hepatitis group)and hepatitis B cirrhosis(cirrhosis group)were selected,healthy volunteers in the same period were set as normal control group.Plasma hsa_circ_0004812 was detected by qRT-PCR.The receiver operating characteristic(ROC)curve was established,to evaluate hsa_circ_0004812 combined with AFP in the diagnosis of liver cancer.Results The expression of hsa_circ_0004812 in patients with HBV related liver cancer was significantly higher than that in normal control group,hepatitis group and liver cirrhosis group(P<0.05).ROC curve showed hsa_circ_0004812 and alpha fetoprotein had high diagnostic efficiency(AUC>0.7)and hsa_circ_0004812 combined with AFP had higher diagnostic efficiency(AUC 0.834-0.853).Conclusion circRNA(hsa_circ_0004812)is a potential biomarker for the diagnosis of HBV related liver cancer,and it has a higher differential ability with AFP.

关 键 词：肝癌 乙型肝炎病毒 环状RNA hsa_circ_0004812 诊断标志物 

分 类 号：R735.7[医药卫生—肿瘤]