西藏林芝桦褐孔菌的化学成分与抗肿瘤活性  

Chemical Composition and Antitumor Activity of Inonotus obliquus from Tibet

作　　者：刘怡[1,2] 刘红丽 程秀峰 张金超 薛蓓 刘振东[1] 汪雯翰[2] LIU Yi;LIU Hongli;CHENG Xiufeng;ZHANG Jinchao;XUE Bei;LIU Zhendong;WANG Wenhan(Tibet Agriculture and Animal Husbandry College,LinZhi 860000,Tibet,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungil Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403 China)

机构地区：[1]西藏农牧学院,西藏林芝860000 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点实验室,上海201403

出　　处：《食用菌学报》2021年第6期126-134,共9页Acta Edulis Fungi

基　　金：西藏农牧学院研究生创新计划资助项目(YJ2020-38);西藏自治区科技计划项目(XZ201801-GA-15)。

摘　　要：通过alamarBlueTM实验比较西藏林芝桦褐孔菌(Inonotus obliquus)与俄罗斯桦褐孔菌乙醇提取物对非小细胞肺癌A549和SPCA-1细胞增殖的抑制作用。运用硅胶柱、MCI GELCHP 20P树脂、Sephadex LH-20葡聚糖凝胶柱等对西藏林芝桦褐孔菌乙醇提取物的乙酸乙酯分部进行分离;对分离的化合物进行结构鉴定,并测定其对A549和SPCA-1细胞增殖的抑制作用。结果表明:与俄罗斯桦褐孔菌乙醇提取物相比,西藏林芝桦褐孔菌乙醇提取物对A549和SPCA-1细胞增殖具有更好的抑制作用。从西藏林芝桦褐孔菌乙醇提取物中分离到10个化合物,分别鉴定为桦褐孔菌醇(H-1)、乌索酸(H-2)、白桦脂醇(H-3)、香草酸(H-4)、丁香酸(H-5)、反式咖啡酸白桦醇脂(H-6)、原儿茶酸(H-7)、没食子酸(H-8)、褐孔菌烷B(H-9)和桦褐孔菌醇A(H-10),其中化合物H-1对A549细胞增殖的抑制效果最好,在300μmol·L^(-1)时抑制率为57.5%;H-2对SPCA-1细胞增殖的抑制效果最好,在300μmol·L^(-1)时抑制率为73.4%。Using the alamarBlueTMassay,Inonotus obliquus from Linzhi,Tibet and Russia were compared for their effects on proliferation of A549and SPCA-1cells in vitro.The ethyl acetate fraction of the ethanol extract of Linzhi I.oliquus was analyzed for chemical composition by silica gel column chromatography,MCI GELCHP 20P,Sephadex LH-20column and other chromatography methods.The isolated chemical compounds were then subjected to structural identification and evaluated for their effects on proliferation of A549and SPCA-1cells in vitro.The results showed that Linzhi I.oliquus showed stronger inhibitory effects on A549and SPCA-1proliferation.A total of 10compounds were isolated from the ethnol extract of Linzhi I.obliquus,and they were identified as follows:betulinol(H-1),ursolic acid(H-2),betulinol(H-3),vanillic acid(H-4),syringic acid(H-5),trans caffeic acid betulinol(H-6),protocatechuic acid(H-7),gallic acid(H-8),inonotusane B(H-9),and inonotsutriol A(H-10).Among these compounds,H-1exhibited the best inhibitory effect on A549cells with an inhibition rate of 57.5%at 300μmol·L^(-1),while H-2showed the best inhibitory effect on SPCA-1cells with an inhibition rate of 73.4%at 300μmol·L^(-1).

关 键 词：桦褐孔菌 桦褐孔菌醇 乌索酸 非小细胞肺癌 

分 类 号：R285[医药卫生—中药学]