MicroRNA-143对白血病细胞系U-937细胞增殖及凋亡的影响  

The Effect of MicroRNA-143 on the Proliferation and Apoptosis of Leukemia Cell Line U-937

作　　者：黄劲龙 方静平 沈建箴[3] HUANG Jin-Long;FANG Jing-Ping;SHEN Jian-Zhen(Department of Hematology,The First Ajfiliated Hospital of Fujian Medical University,Fuzhou 350005,Fujian Province,China;College of Life Science,Fujian Normal University,Fuzhou 350117,Fujian Province,China;Department of Hematology,Union Hospital of Fujian Medical University,Fuzhou 350001,Fujian Province,China)

机构地区：[1]福建医科大学附属第一医院血液科,福建福州350005 [2]福建师范大学生命科学学院,福建福州350117 [3]福建医科大学附属协和医院血液科,福建福州350001

出　　处：《中国实验血液学杂志》2021年第6期1695-1703,共9页Journal of Experimental Hematology

基　　金：国家自然科学基金(41906096);福建医科大学启航基金(2017XQ1060);福建省自然科学基金项目(2019J0102);福建省教育厅中青年教师教育科研项目(JT180082)。

摘　　要：目的:研究microRNA-143(miR-143)在急性髓系白血病(AML)患者中的表达,以及过表达miR-143对AML细胞系U-937细胞增殖和凋亡的影响,验证miR-143和长链非编码RNA MALAT-1之间的靶向关系,探讨miR-143在AML发病过程中可能的作用机制。方法:采用RT-qPCR法检测AML患者骨髓中miR-143的表达。在U-937细胞系中过表达miR-143,采用CCK-8法、克隆形成实验、流式细胞术检测细胞的增殖,通过Hoechst 33258荧光染色、流式细胞术检测细胞的凋亡情况。同时,通过生物信息学,RT-qPCR、双荧光素酶报告基因实验预测及验证miR-143和MALAT-1之间的靶向关系。结果:miR-143在AML患者中的表达量较正常人显著降低。miR-143过表达可抑制U-937细胞的增殖,同时促进细胞凋亡。miR-143结合位点位于MALAT-1 RNA序列上,在miR-143过表达的U-937细胞中MALAT-1的表达下调;沉默miR-143后,MALAT-1表达量升高;然而,沉默MALAT-1后,miR-143的表达未见显著改变。结论:MiR-143在AML患者中低表达。过表达miR-143抑制U-937细胞增殖,促进细胞凋亡。其作用机制可能与靶向调控MALAT-1的表达有关。Objective: To investigate the expression of microRNA-143( miR-143) in patients with acute myeloid leukemia( AML),and the effect of miR-143 over-expression on the proliferation and apoptosis of AML cells. And to verify the targeting relationship between miR-143 and long non-coding RNA MALAT-1. So as to explore the possible regulatory mechanism of miR-143 in the pathogenesis of AML. Methods: The expression of miR-143 in bone marrow cells of AML patients was detected by RT-qPCR. After miR-143 was over-expressed in U-937 cell lines,the proliferation of U-937 cell lines was detected by CCK-8,clone formation assay,and flow cytometry. In addition,cell apoptosis was detected by Hoechst 33258 fluorescence staining and flow cytometry. At the same time,bioinformatics,RT-qPCR and dual luciferase reporter gene assay were used to predicted and verified the targeting relationship between miR-143 and MALAT-1. Results: Expression of miR-143 in AML patients was significantly lower than those in normal controls.Over-expressed miR-143 could inhibit the proliferation of U-937 cells and promote the apoptosis of the cell. The miR-143 binding site was located on the MALAT-1 RNA sequence,and MALAT-1 was down-regulated in U-937 cells after overexpressed miR-143. However,the expression of miR-143 showed no significantly changed after MALAT-1 silencing.Conclusion: Expression of miR-143 in AML patients is lower than that in normal controls. Over-expression of miR-143 can inhibit the proliferation of U-937 cells and promote its apoptosis. And its mechanism may be related to its targe regulation of MALAT-1.

关 键 词：microRNA-143 急性髓系白血病 增殖 凋亡 长链非编码RNA MALAT-1 

分 类 号：R733.71[医药卫生—肿瘤]