固定化酶技术在中药酶抑制剂筛选中的研究现状  

Research status of immobilized enzyme technology in screening of enzyme inhibitors of traditional Chinese medicine

作　　者：安琼 李国峰 郎一帆 杨武亮 陈春兰 张武岗[2] 陈海芳 AN Qiong;LI Guo-feng;LANG Yi-fan;YANG Wu-liang;CHEN Chun-lan;ZHANG Wu-gang;CHEN Hai-fang(Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Jiangxi University of Chinese Medicine,Nanchang 330004,China;National Engineering Center for Manufacturing Technology of Solid Preparation in Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004 [2]江西中医药大学中药固体制剂制造技术国家工程研究中心,江西南昌330004

出　　处：《中草药》2021年第22期7057-7065,共9页Chinese Traditional and Herbal Drugs

基　　金：江西省自然科学基金资助项目(20192BAB205109);国家自然科学基金项目(81903917);江西省教育厅项目(GJJ19067);博士启动基金项目(2018WBZR002)。

摘　　要：固定化酶技术是利用物理或化学方法将游离酶固定在相应的载体上用于筛选酶抑制剂的方法,该方法选择性高、稳定性好。基于疾病相关靶点-生物酶,采用固定化酶技术从中药中筛选疾病相关酶抑制剂,有望成为中药新药研发的重要手段。以固定化酶应用的载体材料为分类综述了近十年来固定化酶技术用于筛选中药中酶抑制剂的研究现状,以期为后续的相关研究提供理论基础。Immobilized enzyme technology is a physical or chemical method to fix free enzyme on the corresponding carrier for screening enzyme inhibitors.This method has high selectivity and high stability.Starting from the different pharmacological effects of traditional Chinese medicine(TCM),using disease-related biological enzymes and combining with immobilized enzyme technology to screen related enzyme inhibitors in TCM,this method may become an important means for new drug research and development.In this paper,the research status of immobilized enzyme in the screening of enzyme inhibitors in TCM in recent ten years was reviewed based on the carrier materials used for immobilized enzyme in order to provide a certain theoretical basis for the subsequent research.

关 键 词：固定化酶 生物酶 中药 载体材料 筛选 酶抑制剂 

分 类 号：R28[医药卫生—中药学] R283.6[医药卫生—中医学]