EGCG通过PI3K-AKT信号通路调控FOXO1磷酸化抑制3T3-L1前脂肪细胞分化  

EGCG Inhibits 3T3-L1 Preadipocyte Differentiation By Regulating the Phosphorylation of FOXO1 through PI3K-AKT Signaling Pathway

作　　者：罗江琼 陈紫君[1] 李延明 袁林颖[2] 周四明 庞科[1] 张莹 LUO Jiangqiong;CHEN Zijun;LI Yanming;YANG Linying;ZHOU Siming;PANG Ke;ZHANG Ying(Affiliated Yongchuan Hospital,Chongqing Medical University,Chongqing 402160,China;Tea Research Institute,Chongqing Academy of Agricultural Sciences,Chongqing 402160,China)

机构地区：[1]重庆医科大学附属永川医院,重庆402160 [2]重庆市农业科学院茶叶研究所,重庆402160

出　　处：《茶叶通讯》2021年第4期717-723,729,共8页Journal of Tea Communication

基　　金：重庆市科委基础与前沿项目(cstc2018jxjl0119);重庆市科委社会民生重点项目(cstc2017shms-zdyf0111);重庆市永川区科委自然基金(Ycstc,2018nb0230)。

摘　　要：为探究EGCG对3T3-L1前脂肪细胞分化的影响及其分子机制,以3T3-L1前脂肪细胞为材料,分别添加不同浓度EGCG(0μM、5μM、10μM、50μM、100μM),通过油红O染色检测EGCG对3T3-L1脂肪细胞分化过程中脂质积累的影响,同时采用RT-PCR和免疫印迹检测不同浓度EGCG对PPARγ、FAS、PI3K、FOXO1的mRNA及蛋白表达的影响。油红O染色结果表明,EGCG可抑制3T3-L1前脂肪细胞分化,阻碍脂质积累,并具有浓度效应,EGCG浓度越高抑制作用越明显;EGCG能够直接抑制PPARγ和FAS的表达,同时抑制P-AKT、P-PI3K以及P-FOXO1的表达。表明EGCG通过介导PI3K/AKT-FOXO1级联信号通路,下调具有促进前脂肪细胞分化功能的PPARγ和FAS的表达,从而抑制前脂肪细胞分化,阻碍脂肪生成。To explore the effect of EGCG on the differentiation of 3T3-L1 preadipocytes and its molecular mechanism.Different concentrations of EGCG(0μM,5μM,10μM,50μM,100μM)were added to 3T3-L1 preadipocytes,respectively.The effects of EGCG on the lipid accumulation during the period of adipocytes differentiation were detected by oil red O staining.Meanwhile,the RT-PCR and Western blot were conducted to detect the effects of different concentrations of EGCG on the mRNA and protein expression levels of PPARγ,FAS,PI3K and FOXO1.The results of oil red O showed that EGCG inhibited the differentiation of 3T3-L1 preadipocyte and hinder lipid accumulation,and had a concentration effect,the higher the concentration of EGCG,the more obvious the inhibition effect was;EGCG could directly inhibit PPARγAnd FAS,and inhibit the expression of P-AKT、P-PI3K and P-FOXO1.These results suggest that EGCG down-regulate the expression of PPARγand FAS which have the function of promoting preadipocyte differentiation by mediating PI3K/AKT-FOXO1 cascade signaling pathway,thereby inhibiting preadipocyte differentiation and hindering adipogenesis.

关 键 词：3T3-L1 细胞分化 EGCG PI3K/AKT通路 FOXO1 茶叶 

分 类 号：S571.1[农业科学—茶叶生产加工]