DFO对糖尿病大鼠心肌缺血再灌注损伤的保护作用  

The protective effect of DFO on myocardial ischemia-reperfusion injury in diabetic rats

作　　者：马宁[1] 陈宇[1] 黄一丹[1] 杨龙[1] 吴建江[1] MA Ning;Chen Yu;HUANG Yidan;YANG Long;WU Jianjiang(Department of Anesthesiology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院麻醉科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2021年第12期1332-1335,1345,共5页Journal of Xinjiang Medical University

基　　金：新疆医科大学临床医学高峰学科校内配套经费资助项目(33-0104006020801#);新疆维吾尔自治区重点实验室开放课题(2019D04019)。

摘　　要：目的探讨去铁胺(DFO)在七氟醚后处理(SPostC)的心肌保护作用中,与线粒体呼吸功能以及低氧诱导因子-1α(HIF-1α)之间的关系。方法依照随机数字表法将80个Langendorff离体灌注模型成功的糖尿病大鼠心脏分成5组(每组16只):对照组(C组)、缺血再灌注组(I/R组)、七氟醚后处理组(SPostC组)、去铁胺+七氟醚后处理组(DFO+SPostC)、去铁胺+七氟醚后处理+HIF-1α抑制剂二甲氧基雌二醇(2ME2)组(DFO+SPostC+2ME2)。检测线粒体呼吸功能、线粒体呼吸酶活性、Western blot法测定HIF-1α蛋白表达水平以及线粒体超微结构。结果与C组比较,其余各组呼吸功能及酶活性参数变化差异有统计学意义(P<0.05),与I/R组相比,DFO+SPostC组中HIF-1α表达明显提高,线粒体3态呼吸(State3)、呼吸控制率(RCR)、烟酰胺腺嘌呤二核苷酸氧化酶(NADHO)、细胞色素c氧化酶(CcO)和琥珀酸氧化酶(SUCO)的表达明显上升,但是在DFO+SPostC+2ME2组中HIF-1α表达受抑制(P<0.05)。结论去铁胺处理导致HIF-1α水平升高使线粒体功能恢复,可能是七氟醚后处理减轻糖尿病大鼠心肌缺血再灌注损伤的关键点。Objective To investigate the relationship between the myocardial protective effect of deferoxamine(DFO)sevoflurane postcorditioning,mitochondrial respiratory function and HIF-1α.Methods 80 isolated diabetic rat hearts perfused in a Langendorff apparatus were randomly divided into 5 groups(n=16)with using a random number table:the control group(C group),ischemia-reperfusion group(I/R group),sevoflurane postconditioning group(SPostC group),deferoxamine pretreatment combined with the sevoflurane postconditioning group(DFO+SPostC group)and inhibitor group(DFO+SPostC+2 ME2 group).Mitochondrial respiratory function and enzyme activity were measured by oxygen electrode,and detection of HIF-1αprotein levels was measured by Western blot.The ultrastructure of mitochondria was observed by electron microscope.Results Compared with the group C,the changes of mitochondrial respiratory function and enzyme activity in the other groups were statistically significant(P<0.05).Compared with I/R group,HIF-1αexpression was significantly increased in DFO+SPostC group.State3,RCR,NADHO,CcO and SUCO activity were higher,but in the DFO+SPostC+2 ME2 group,the effect of HIF-1αwas inhibited(P<0.05).Conclusion In diabetic rats,deferoxamine pretreatment resulted in HIF-1αlevel was increased that promote the recovery of mitochondrial function.,It may be the key point for sevoflurane postconditioning to alleviate myocardial ischemia-reperfusion injury in diabetic patients.

关 键 词：七氟醚后处理 低氧诱导因子-1Α 线粒体功能 

分 类 号：R575[医药卫生—消化系统]