响应面法优化紫花芸豆凝集素的反胶束萃取工艺  

Optimization of Reverse Micelle Extraction of Lectin from Purple Kidney Bean by Response Surface Methodology

作　　者：符家良 李春莲 张红杨 黄齐林 阮长青[1,2,3,4] 张东杰 FU Jialiang;LI Chunlian;ZHANG Hongyang;HUANG Qilin;RUAN Changqing;ZHANG Dongjie(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319;Key Laboratory of Agro-products Processing and Quality Safety of Heilongjiang Province,Daqing 163319;National Coarse Cereals Engineering Research Center,Daqing 163319;Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319 [2]黑龙江省农产品加工与质量安全重点实验室,大庆163319 [3]国家杂粮工程技术研究中心,大庆163319 [4]黑龙江省杂粮加工及质量安全工程技术研究中心,大庆163319

出　　处：《食品工业》2021年第11期152-156,共5页The Food Industry

基　　金：国家重点研发计划(编号:2018YFE0206300);黑龙江省自然基金研究团队项目(编号:TD2020C003);黑龙江省杂粮产业技术协同创新体系杂粮食品加工技术协同创新岗、优势特色学科资助项目(编号:黑教联[2018]4号);黑龙江八一农垦大学“三横三纵”科研创新团队项目(编号:TDJH201806)。

摘　　要：利用反胶束萃取法对紫花芸豆凝集素进行分离纯化,经响应面优化试验得到最佳参数:前萃取离子强度50 mmol/L,萃取时间20 min,前萃取pH 5.8, AOT浓度60 mmol/L,后萃取pH 9.0,后萃取离子强度530 mmol/L。在该条件下,蛋白回收率为82.66%,纯化倍数为9.65倍,试验证明反胶束萃取可用于紫花芸豆凝集素的分离纯化并保留其活性。The lectin of Purple kidney bean was isolated and purified by reverse micelle extraction, and its physical and chemical properties were determined. The optimal parameters obtained by the response surface optimization test were as follows: pre-extraction ion concentration was 50 mmol/L, extraction time was 20 min, pre-extraction pH was 5.8, AOT concentration was 60 mmol/L, post-extraction pH was 9.0, and post-extraction ion concentration was 530 mmol/L. Under this condition, the protein recovery rate was 82.66%, and the purification rate was 9.65 times, which proved that reverse micelle extraction could be used to isolate and purify lectin from purple kidney bean and retain its activity.

关 键 词：紫花芸豆 凝集素 反胶束萃取 

分 类 号：TS214.9[轻工技术与工程—粮食、油脂及植物蛋白工程]