GPR75受体介导20-HETE信号通路对雄激素非依赖性前列腺癌细胞增殖、迁移、侵袭的影响  

Effect of GPR75 receptor mediated 20-HETE signaling pathway on proliferation,migration and invasion of androgen independent prostate cancer cells

作　　者：赵华[1] 姚硕 刘丽 Zhao Hua;Yao Shuo;Liu Li(Department of Endoscopy,Tianjin Beichen Hospital,Tianjin 300400,China;Community Health Service Center of Shuangjie Town,Tianjin 300400,China)

机构地区：[1]天津市北辰医院内镜科,300400 [2]天津市北辰区双街镇社区卫生服务中心,300400

出　　处：《国际泌尿系统杂志》2021年第6期968-972,共5页International Journal of Urology and Nephrology

摘　　要：目的探讨G蛋白偶联受体75(GPR75)受体介导20-羟二十烷四烯酸(20-HETE)信号通路对雄激素非依赖性前列腺癌细胞增殖、迁移、侵袭的影响。方法体外培养PC-3雄激素非依赖性前列腺癌细胞系,未经干预为对照组,细胞转染敲减GPR75受体为shGPR75组,20-HETE干预为20-HETE组,细胞转染敲减GPR75联合20-HETE干预为shGPR75+20-HETE组。采用Western blot检测各组GPR75表达情况,采用四唑盐比色法(MTT)观察PC-3细胞增殖情况,采用Transwell检测PC-3细胞侵袭能力,采用划痕实验检测PC-3细胞迁移能力。结果对照组GPR75蛋白表达量为(1.04±0.13),shGPR75组为(0.42±0.03),20-HETE组为(1.27±1.20),shGPR75+20-HETE组为(0.68±0.07),四组GPR75蛋白表达水平整体比较差异有统计学意义(P<0.05)。对照组细胞存活率为(76.30±10.30)%,shGPR75组为(40.33±4.60)%,20-HETE组为(88.50±12.61)%,shGPR75+20-HETE组为(54.38±6.70)%,四组PC-3细胞存活率整体比较,差异具有统计学意义(F=30.269,P<0.05)。Transwell法结果显示,对照组侵袭能力为(107.30±15.39)个,shGPR75组为(62.30±7.20)个,20-HETE组为(123.57±18.90)个,shGPR75+20-HETE组为(80.11±10.61)个,四组侵袭能力整体比较,差异有统计学意义(P<0.05)。划痕实验显示,对照组迁移能力为(142.50±20.33)个,shGPR75组为(86.24±10.60)个,20-HETE组为(170.93±24.78)个,shGPR75+20-HETE组为(113.65±16.01)个,四组差异有统计学意义(P<0.05)。结论20-HETE可促进雄激素非依赖性前列腺癌细胞增殖、侵袭、迁移,通过抑制GPR75受体可降低20-HETE的促癌作用。Objective To investigate the effect of G protein coupled receptor 75(GPR75)receptor mediated 20-hydroxyeicosatetraenoic acid(20-HETE)signaling pathway on proliferation,migration and invasion of androgen independent prostate cancer cells.Methods PC-3 androgen-independent prostate cancer cell lines were cultured in vitro,and divided into four groups,control group(no treatment),shGPR75 group(transfection of knockdown GPR75 receptor),20-HETE group(20-HETE intervention)and shGPR75+20-HETE group(transfection of knockdown GPR75 receptor+20-HETE intervention).Then various indexes were detected using different methods,including expression of GPR75 by Western blot,proliferation of PC-3 cells by Methyl thiazolyl tetrazolium(MTT)assay,invasion ability of PC-3 cells by Transwell chamber assay,and migration ability of PC-3 cells by scratch test.Results The expression level of GPR75 protein was(1.04±0.13)in control group,(0.42±0.03)in shGPR75 group,(1.27±1.20)in 20-HETE group,and(0.68±0.07)in shG-PR75+20-HETE group,with statistical difference(P<0.05).The survival rate of PC-3 cell was(76.30±10.30)in control group,(40.33±4.60)in shGPR75 group,(88.50±12.61)in 20-HETE group,and(54.38±6.70)in shGPR75+20-HETE group,with statistical difference(P<0.05).Transwell chamber assay showed that the invasion ability of PC-3 cells was(107.30±15.39)in control group,(62.30±7.20)in shGPR75 group,(123.57±18.90)in 20-HETE group,and(80.11±10.61)in shGPR75+20-HETE group,with statistical difference(P<0.05).The migration ability of PC-3 cells was(142.50±20.33)in control group,(86.24±10.60)in shGPR75 group,(170.93±24.78)in 20-HETE group,and(113.65±16.01)in shGPR75+20-HETE group,with statistical difference(P<0.05).Conclusions 20-HETE can promote the proliferation,invasion and migration of androgen non-replacement prostate cancer cells,which may be related to the reduction of the cancer-promoting effect of 20-HETE by inhibiting the GPR75 receptor.

关 键 词：前列腺肿瘤 G蛋白偶联受体75 20-羟二十烷四烯酸 细胞运动 

分 类 号：R737.25[医药卫生—肿瘤]