基于多肽识别的电化学生物传感技术  

Recent Advances in Peptide-Based Electrochemical Biosensor

作　　者：张晗[1,2] 丁家旺 秦伟[1] Han Zhang;Jiawang Ding;Wei Qin(CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research(YIC),Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院海岸带环境过程与生态修复重点实验室中国科学院烟台海岸带研究所,烟台264003 [2]中国科学院大学,北京100049

出　　处：《化学进展》2021年第10期1756-1765,共10页Progress in Chemistry

基　　金：国家自然科学基金面上项目(No.41876108);山东省泰山学者人才项目(No.tsqn201909163,tspd20181215)资助。

摘　　要：多肽具有分子量小、易于合成、生物兼容性好、稳定性高及序列灵活多样等优点。因此,多肽作为新型生物识别元件,已被广泛应用于生物传感器的构建。电化学分析灵敏度高、准确度好、设备简单、检测范围广且易于操作。本文介绍了基于多肽识别的电化学生物传感器技术,包括多肽的修饰与固定化、多肽与待测物的识别及检测原理;综述了近五年多肽电化学生物传感器对重金属离子、小分子、蛋白质、细菌和病毒的检测;展望了肽基电化学生物传感器的发展趋势。Peptides with unique features such as small molecular weight,ease synthesis,good biocompatibility,high stability and versatile sequences,have received increasing interest as recognition elements for biosensors.Electrochemical analysis has wide applications because of its high sensitivity,good accuracy,simple equipment,wide detection range and easy to use.The peptide-based electrochemical biosensors can be used in many fields including environment monitoring,biomedicine and food detection.In this review,we introduce the peptide-based electrochemical biosensors for detection heavy metal ions,small-molecules,proteins,pathogenic bacteria and viruses.The strategies for peptide modification and immobilization are summarized.We also describe the properties and sensing mechanisms of peptide-based electrochemical biosensors.Sensing strategies especially those based on target-binding induced combination,digestion and phosphorylation of peptides have been discussed.Lastly,the current problems and prospect of the research on peptide-based electrochemical biosensor are discussed and prospected.

关 键 词：多肽 生物传感 电化学分析 识别分子 检测 

分 类 号：O657.1[理学—分析化学] TP212.3[理学—化学]