基于UHPLC-Q-TOF/MS的地榆化学成分分析及大鼠体内代谢研究  

Identification of Chemical Composition of Sanguisorba officinalis L.and Its Major Metabolites in Rats by UHPLC-Q-TOF/MS

作　　者：裴玉琼[1] 徐坠成 王艳天 顾欣 盛先杰 徐艺[2] 缪志伟[3] PEI Yu-qiong;XU Zhui-cheng;WANG Yan-tian;GU Xin;SHENG Xian-jie;XU Yi;MIAO Zhi-wei(School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China;The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China;Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, 215600, China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]南京中医药大学附属医院,江苏南京210029 [3]南京中医药大学附属张家港医院,江苏张家港215600

出　　处：《南京中医药大学学报》2021年第6期897-907,共11页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药科技发展计划(RC201903);江苏省“333高层次人才培养工程”科研项目(LGY2018060);苏州市科技发展计划(SYS2020056);南京中医药大学自然科学基金(XZR2020059)。

摘　　要：目的采用超高效液相色谱和-四级杆串联飞行时间质谱(UHPLC-Q-TOF/MS)法分析地榆水提物在大鼠血清、尿液及粪便中的原型成分及代谢产物,探讨地榆中活性成分在大鼠体内的代谢途径。方法采用Thermo Acclaim^(TM) RSLC 120 C_(18)色谱柱(3.0 mm×100 mm,2.2μm),以0.1%甲酸水溶液(A)-乙腈(B)为流动相进行梯度洗脱,流速0.4 mL·min^(-1),进样量3μL,柱温40℃。运用电喷雾离子源(ESI),扫描范围m/z 50~1500,正、负离子模式采集数据。通过比较灌胃地榆水提物后大鼠血清、尿液和粪便样品和空白组样品的差异,对原型成分及其代谢产物进行鉴定。结果灌胃给予地榆水提物后,从大鼠血清、尿液及粪便中鉴定出69个外源性成分,包括24个原型成分和45个代谢产物。其中24个原型成分包括16个酚类成分、7个三萜类成分及1个单萜烯醇苷。地榆水提物在大鼠体内的代谢途径主要包括还原、甲基化、脱水、氧化、硫酸化及葡萄糖醛酸结合等。结论所鉴定出的原型成分和代谢产物可为进一步解析地榆体内代谢过程,阐明其药效物质基础提供实验依据。OBJECTIVE To analyze the prototype components and metabolites of aqueous extract of Sanguisorba officinalis L.by UHPLC-Q-TOF/MS in feces,urine and plasma of rats and explore its metabolic pathways.METHODS Chromatographic separation was performed on the Thermo Acclaim^(TM) RSLC 120 C_(18) column(3.0 mm×100 mm,2.2μm).The mobile phase was consisted of 0.1%formic acid in water(solvent A)and acetonitrile(solvent B).The sample injection volume was 3μL and the flow rate was set to 0.4 mL·min-1.The column temperature was maintained at 40℃.The detection was carried out over a mass range of m/z 50-1500 and equipped with an electrospray ionization(ESI)source operating in positive mode and negative mode.Chemical composition on Sanguisorba officinalis L.and its major metabolites were identified by comparing the differences in feces,urine and plasma between adminstration samples and blank samples in rats.RESULTS After intragastric administration of the aqueous extract of Sanguisorba officinalis L.,69 exogenous components were identified from rat plasma,urine and feces,including 24 prototype components and 45 metabolites.24 prototype components included 16 phenolics,7 triterpenes,and a monoterpene glycoside.The metabolic pathways of the aqueous extract of Sanguisorba officinalis L.in rats mainly included reduction,methylation,dehydration,oxidation,sulfation,and glucuronidation.CONCLUSION The identified prototype drugs and the metabolites can supply a clear material basis for the further study of efficacy of Sanguisorba officinalis L..

关 键 词：地榆 UHPLC-Q-TOF/MS 原型成分 代谢产物 

分 类 号：R284[医药卫生—中药学]