M2型肿瘤相关巨噬细胞在胰腺癌组织中的表达及其与预后的关系  

Expression of M2 Tumor-Associated Macrophages in Pancreatic Cancer Tissue and Its Relationship to Prognosis

作　　者：陈凯[1] 廖芮 杨洪吉[1] 朱世凯[1] 杨冲 董丹丹[2] 李科[2] 李有赞 Chen Kai;Liao Rui;Yang Hongji;Zhu Shikai;Yang Chong;Dong Dandan;Li Ke;Li Youzan(Organ Transplantation Center,Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospi-tal,Chengdu 610072,Sichuan,China;Department of Pathology,Sichuan Academy of Medical Sciences&Sichuan Provincial People’s Hospital,Chengdu 610072,Sichuan,China;School of Clinical Medicine,Southwest Medical Univer-sity,Luzhou 646099,Sichuan,China)

机构地区：[1]四川省人民医院,电子科技大学器官移植中心,成都610072 [2]四川省人民医院,电子科技大学病理科,成都610072 [3]西南医科大学临床医学院,四川泸州646099

出　　处：《肿瘤预防与治疗》2021年第11期1035-1041,共7页Journal of Cancer Control And Treatment

基　　金：四川省卫健委科研课题(编号:19PJ135)。

摘　　要：目的:探讨CD68^(+)、CD163^(+)标记的M2型肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)在胰腺癌组织中的表达及对胰腺癌患者预后的影响。方法:收集2017年1月至2019年12月期间在四川省人民医院行手术治疗并经术后病理确诊的208例胰腺导管腺癌患者的临床资料,应用免疫组织化学检测胰腺导管腺癌组织CD68、CD163蛋白表达情况,分析其与肿瘤临床病理参数的相关性,并对影响患者预后的多个临床病理因素进行单因素及多因素分析。结果:CD68及CD163的蛋白表达主要定位于胞质,两者染色均呈为棕黄或棕褐色,CD68和CD163的阳性表达率分别为84.1%(175/208)和76.0%(158/208)。CD68的阳性表达与肿瘤临床分期密切相关,CD163的阳性表达与肿瘤临床分期、肿瘤大小密切相关(P<0.05)。CD68^(+)和CD163^(+)双表达与肿瘤临床分期、肿瘤大小密切相关,临床分期越晚、肿瘤越大其双阳性表达率也越高(P<0.05)。多因素分析显示,肿瘤大小>2cm、临床分期Ⅲ~Ⅳ期及CD68阳性表达是影响胰腺导管腺癌患者预后生存的独立危险因素(P<0.05)。结论:M2型TAMs在胰腺导管腺癌组织中广泛浸润,M2型TAMs可作为胰腺导管腺癌预后的潜在分子标志物。Objective:To investigate the expression of M2 tumor-associated macrophages in pancreatic cancer and its relationship to prognosis.Methods:A total of 208 patients who underwent surgical resection and were pathologically diagnosed as pancreatic ductal adenocarcinoma in the department of hepatobiliary pancreaticosplenic surgery in Sichuan Provincial People's Hospital from January 2017 to December 2019 were enrolled.The expression of CD68 and CD163 proteins in pancreat-ic ductal adenocarcinoma were detected by immunohistochemical staining,and its correlation to clinicopathological parameters were analyzed.Cox proportional hazard models were used to univariately and multivariately analyze the prognostic factors of pancreatic cancer.Results:CD68 and CD163 proteins were mainly expressed in the cytoplasm,with positive expression rates of 84.1%(175/208)and 76.0%(158/208),respectively.The sliced samples of CD68 and CD163 were buffy and sepia,respectively.The positive expression of CD68 was closely related to clinical stage(P<0.05),and that of CD163 was closely related to clinical stage and tumor size(P<0.05).The concurrent expression of CD68 and CD163 was also positively related to clinical stage and tumor size(P<0.05);the concurrent expression rate increased as clinical stage advanced as well as the tumor size increased.Multivariate analysis showed that tumor size>2 cm,clinical stage III-IV and CD68 positive expression were independent risk factors for the survival of patients with pancreatic ductal adenocarcinoma(P<0.05).Conclusion:M2 tumor-associated macrophages are widely infiltrated in pancreatic ductal adenocarcinoma.They can be used as a potential molecular marker for the prognosis of pancreatic ductal adenocarcinoma.

关 键 词：胰腺癌 肿瘤相关巨噬细胞 CD68 CD163 预后 

分 类 号：R735.9[医药卫生—肿瘤]